Large-Scale Proteomic Analysis of the Human Spliceosome

  1. Juri Rappsilber1,
  2. Ursula Ryder2,
  3. Angus I. Lamond2, and
  4. Matthias Mann1,3
  1. 1Protein Interaction Laboratory in the Center of Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark; 2Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland, United Kingdom

Abstract

In a previous proteomic study of the human spliceosome, we identified 42 spliceosome-associated factors, including 19 novel ones. Using enhanced mass spectrometric tools and improved databases, we now report identification of 311 proteins that copurify with splicing complexes assembled on two separate pre-mRNAs. All known essential human splicing factors were found, and 96 novel proteins were identified, of which 55 contain domains directly linking them to functions in splicing/RNA processing. We also detected 20 proteins related to transcription, which indicates a direct connection between this process and splicing. This investigation provides the most detailed inventory of human spliceosome-associated factors to date, and the data indicate a number of interesting links coordinating splicing with other steps in the gene expression pathway.

Footnotes

  • 3 Corresponding author.

  • E-MAIL mann{at}bmb.sdu.dk; FAX 45 6593 3929.

  • Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.473902. Article published online before print in July 2002.

    • Received May 29, 2002.
    • Accepted June 14, 2002.
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