Studies of Metaphase and Interphase Chromosomes Using Fluorescence In Situ Hybridization

B.J. Trask; S. Allen; H. Massa; A. Fertitta; R. Sachs; G. van den Engh; M. Wu

doi:10.1101/SQB.1993.058.01.084

Studies of Metaphase and Interphase Chromosomes Using Fluorescence In Situ Hybridization

^*Department of Molecular Biotechnology, University of Washington, Seattle, Washington 98195; ^†Human Genome Center, Lawrence Livermore National Laboratory, Livermore, California 94550; ^‡Department of Mathematics, University of California, Berkeley, California 94720

This extract was created in the absence of an abstract.

Excerpt

The packaging of chromosomes in metaphase and interphase chromosomes can be directly studied by means of fluorescence in situ hybridization (FISH). With this method a fluorescent signal is produced at the site of specific DNA sequences (probes). With different probe modification strategies, different DNA sequences can be localized with respect to one another in chromosomes or nuclei. In this paper, we briefly review the technique, because of its potential impact on the inference of the organization of native, untreated chromatin from in situ results. We describe how the proximity of sequences in interphase and metaphase chromosomes correlates with genomic distance (the distance defined in base pairs along the linear DNA molecule). We contrast the resolution that can be obtained in metaphase and interphase chromosomes. Finally, we discuss the impact of these findings on the combined use of in situ hybridization and specific DNA sequences to detect chromosome abnormalities.

FISH

FISH...