Abstract
A novel human homologue (GCMB) of the Drosophila glial cells missing gene (dGCM) was isolated using RACE. GCMB contained a gcm motif sequence and a nuclear targeting sequence similar to that of dGCM and mouse GCMb. Homology searches indicated that GCMB was located within chromosome 6p24.2. Transcripts of GCMB were detected by means of RT-PCR in fetal brain, normal adult kidney, 3/3 medulloblastomas, 1/3 gliomas and 4/8 non-neuroepithelial tumor cell lines. Our data suggest that humans have two homologues of gcm like mice and that human gcm genes form a novel family which may function not only during fetal development but also in the postnatal or pathological stage.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Southern
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Brain / embryology
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Brain / metabolism*
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Chromosomes, Human, Pair 6 / genetics
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Cloning, Molecular*
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DNA-Binding Proteins
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Drosophila Proteins
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Drosophila melanogaster / genetics
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Expressed Sequence Tags
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Gene Expression*
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Gene Library
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Humans
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Kidney / metabolism
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Molecular Sequence Data
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Neoplasms, Neuroepithelial / metabolism
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Neuropeptides / chemistry
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Neuropeptides / genetics*
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Polymerase Chain Reaction
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
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Trans-Activators / chemistry
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Trans-Activators / genetics*
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Transcription Factors
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Tumor Cells, Cultured
Substances
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DNA-Binding Proteins
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Drosophila Proteins
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GCM protein, Drosophila
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Neuropeptides
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Trans-Activators
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Transcription Factors