Abstract
The transforming growth factor (TGF)-beta superfamily comprises more than 35 structurally related genes that have been implicated in embryonic induction and morphogenesis. Different superfamily members may have distinct regulatory roles in tooth development and maintenance. Degenerate primer sets derived from the highly conserved carboxy terminal region of the TGF-beta superfamily were used for reverse transcriptase polymerase with poly(A)+ RNA from the rat incisor pulp as a template. TGF-beta superfamily members expressed in the pulp with known potential to differentiate into odontoblasts and to form dentine were identified. Nucleotide-sequence analysis of the amplified cDNAs identified those encoding activin-betaB; bone morphogenic protein (BMP)-2, -4, -7 and -8; growth/differentiation factor (GDF)-1, -5 and -6; and glial cell line-derived neurotrophic factor. In addition, Northern blot analysis detected TGF-beta1 -beta2 and -beta3; activin-betaA; BMP-6 and GDF-7 mRNA transcripts in the pulp. Coordinated expression of TGF-beta superfamily members in pulp may be critical in tooth development and repair.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Activins*
-
Animals
-
Blotting, Northern
-
Bone Marrow / metabolism
-
Bone Morphogenetic Protein 2
-
Bone Morphogenetic Protein 4
-
Bone Morphogenetic Protein 7
-
Bone Morphogenetic Proteins / analysis
-
Bone Morphogenetic Proteins / genetics
-
Cell Differentiation / genetics
-
Dental Pulp / metabolism*
-
Dentin / cytology
-
Dentin / metabolism
-
Gene Expression Regulation
-
Growth Differentiation Factor 1
-
Growth Differentiation Factor 5
-
Growth Differentiation Factor 6
-
Growth Substances / analysis
-
Growth Substances / genetics
-
Incisor
-
Intercellular Signaling Peptides and Proteins*
-
Nerve Growth Factors
-
Nerve Tissue Proteins / analysis
-
Nerve Tissue Proteins / genetics
-
Odontoblasts / cytology
-
Odontoblasts / metabolism
-
Odontogenesis / genetics
-
Oligopeptides*
-
Peptides / analysis
-
Peptides / genetics
-
Rats
-
Rats, Inbred LEC
-
Transforming Growth Factor beta / analysis*
-
Transforming Growth Factor beta / genetics
Substances
-
Bmp2 protein, rat
-
Bmp4 protein, rat
-
Bmp7 protein, rat
-
Bone Morphogenetic Protein 2
-
Bone Morphogenetic Protein 4
-
Bone Morphogenetic Protein 7
-
Bone Morphogenetic Proteins
-
Gdf1 protein, rat
-
Gdf6 protein, rat
-
Growth Differentiation Factor 1
-
Growth Differentiation Factor 5
-
Growth Differentiation Factor 6
-
Growth Substances
-
Intercellular Signaling Peptides and Proteins
-
Nerve Growth Factors
-
Nerve Tissue Proteins
-
Oligopeptides
-
Peptides
-
Transforming Growth Factor beta
-
activin B
-
Activins
-
myelopeptides