Abstract
Overlapping cDNA clones from human heart and melanoma libraries were used to establish the 1587-residue sequence of a novel protein (LTBP-4) belonging to the family of extracellular microfibrillar proteins which also bind transforming growth factor-beta. LTBP-4 consists of 20 EG modules, 17 of them with a consensus sequence for calcium binding, 4 TB modules with 8 cysteines and several proline-rich regions. Northern blots demonstrated a single 5 kb mRNA which is highly expressed in heart but also present in skeletal muscle, pancreas, placenta and lung. The modular structure predicts that LTBP-4 should be a microfibrillar protein which probably also binds TGF-beta.
MeSH terms
-
Adaptor Proteins, Signal Transducing*
-
Amino Acid Sequence
-
Base Sequence
-
Blotting, Northern
-
Calcium / metabolism
-
Carrier Proteins / chemistry*
-
Carrier Proteins / genetics
-
Carrier Proteins / metabolism
-
Cloning, Molecular
-
Extracellular Matrix / chemistry
-
Gene Expression
-
Humans
-
Intracellular Signaling Peptides and Proteins*
-
Latent TGF-beta Binding Proteins
-
Melanoma / chemistry
-
Molecular Sequence Data
-
Muscle Proteins / chemistry*
-
Myocardium / chemistry
-
Protein Binding
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
Sequence Alignment
-
Sequence Analysis, DNA
-
Transforming Growth Factor beta / metabolism
Substances
-
Adaptor Proteins, Signal Transducing
-
Carrier Proteins
-
Intracellular Signaling Peptides and Proteins
-
LTBP4 protein, human
-
Latent TGF-beta Binding Proteins
-
Muscle Proteins
-
RNA, Messenger
-
Transforming Growth Factor beta
-
Calcium