Abstract
The COX17 gene of Saccharomyces cerevisiae codes for a cytoplasmic protein essential for the expression of functional cytochrome oxidase. This protein has been implicated in targeting copper to mitochondria. To determine if Cox17p is present in mammalian cells, a yeast strain carrying a null mutation in COX17 was transformed with a human cDNA expression library. All the respiratory competent clones obtained from the transformations carried a common cDNA sequence with a reading frame predicting a product homologous to yeast Cox17p. The cloning of a mammalian COX17 homolog suggests that the encoded product is likely to function in copper recruitment in eucaryotic cells in general. Its presence in humans provides a possible target for genetically inherited deficiencies in cytochrome oxidase.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Carrier Proteins
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Cation Transport Proteins*
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Copper / metabolism*
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Copper Transport Proteins
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DNA, Complementary / isolation & purification
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Electron Transport Complex IV / metabolism
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Escherichia coli
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Genetic Complementation Test
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HeLa Cells
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Humans
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Mitochondria / metabolism*
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Molecular Chaperones
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Molecular Sequence Data
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Mutation
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Plasmids
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Proteins / genetics*
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Restriction Mapping
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
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Transformation, Genetic
Substances
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COX17 protein, S cerevisiae
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COX17 protein, human
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Carrier Proteins
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Cation Transport Proteins
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Copper Transport Proteins
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DNA, Complementary
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Molecular Chaperones
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Proteins
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Saccharomyces cerevisiae Proteins
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Copper
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Electron Transport Complex IV