Cloning of cDNA encoding human rapsyn and mapping of the RAPSN gene locus to chromosome 11p11.2-p11.1

Genomics. 1996 Aug 1;35(3):613-6. doi: 10.1006/geno.1996.0409.

Abstract

We have isolated and sequenced cDNA clones for the human 43-kDa acetylcholine receptor-associated protein rapsyn. The cDNA encodes a 412-amino-acid protein that has a predicted molecular mass of 46,330 Da and shows 96% sequence identity with mouse rapsyn. Analysis of PCR amplifications, first from somatic cell hybrids and subsequently from radiation hybrids, localizes the human RAPSN gene locus to chromosome 11p11.2-p11.1 in close proximity to ACP2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11*
  • Cloning, Molecular
  • DNA, Complementary
  • Humans
  • Hybrid Cells
  • Mice
  • Molecular Sequence Data
  • Muscle Proteins / genetics*
  • Receptors, Nicotinic / genetics*

Substances

  • DNA, Complementary
  • Muscle Proteins
  • Receptors, Nicotinic
  • peripheral membrane protein 43K

Associated data

  • GENBANK/Z33905