Abstract
The Notch gene of Drosophila encodes a large transmembrane protein involved in cell-cell interactions and cell fate decisions in the Drosophila embryo. We report here the isolation of cDNA clones encompassing the full-length coding sequence of Notch-1, a mouse homolog of Drosophila Notch. The predicted amino acid sequence of the Notch-1 protein retains all of the conserved amino acid motifs of Notch and the other vertebrate Notch homologs. The cDNA sequence predicts a 2531-amino-acid protein containing a signal peptide, 36 epidermal growth factor-like repeats, 3 Notch/lin-12 repeats, a transmembrane domain, and 6 cdc10/ankyrin repeats. The Notch-1 gene was localized to the proximal portion of mouse chromosome 2 by mapping with an interspecific backcross panel.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Chromosome Mapping*
-
Cloning, Molecular
-
DNA
-
Drosophila
-
Drosophila Proteins
-
Female
-
Insect Hormones / genetics*
-
Male
-
Membrane Proteins / genetics*
-
Mice
-
Mice, Inbred C57BL
-
Molecular Sequence Data
-
Receptor, Notch1
-
Receptors, Cell Surface*
-
Receptors, Notch
-
Sequence Homology, Amino Acid
-
Transcription Factors*
Substances
-
Drosophila Proteins
-
Insect Hormones
-
Membrane Proteins
-
N protein, Drosophila
-
Notch1 protein, mouse
-
Receptor, Notch1
-
Receptors, Cell Surface
-
Receptors, Notch
-
Transcription Factors
-
DNA
Associated data
-
GENBANK/L14299
-
GENBANK/L14300
-
GENBANK/L14301
-
GENBANK/U04288
-
GENBANK/U04289
-
GENBANK/U04290
-
GENBANK/U04291
-
GENBANK/U04292
-
GENBANK/U04293
-
GENBANK/Z11886