Single-cell epigenomics: powerful new methods for understanding gene regulation and cell identity

Stephen J Clark; Heather J Lee; Sébastien A Smallwood; Gavin Kelsey; Wolf Reik

doi:10.1186/s13059-016-0944-x

Single-cell epigenomics: powerful new methods for understanding gene regulation and cell identity

Genome Biol. 2016 Apr 18:17:72. doi: 10.1186/s13059-016-0944-x.

Authors

Stephen J Clark¹, Heather J Lee^{2

3}, Sébastien A Smallwood^{1

4}, Gavin Kelsey^{1

5}, Wolf Reik^{1

6

5

7}

Affiliations

¹ Epigenetics Programme, Babraham Institute, Cambridge, CB22 3AT, UK.
² Epigenetics Programme, Babraham Institute, Cambridge, CB22 3AT, UK. heather.lee@babraham.ac.uk.
³ Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK. heather.lee@babraham.ac.uk.
⁴ Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH 4058, Basel, Switzerland.
⁵ Centre for Trophoblast Research, University of Cambridge, Cambridge, CB2 3EG, UK.
⁶ Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK.
⁷ Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, CB2 3EG, UK.

Abstract

Emerging single-cell epigenomic methods are being developed with the exciting potential to transform our knowledge of gene regulation. Here we review available techniques and future possibilities, arguing that the full potential of single-cell epigenetic studies will be realized through parallel profiling of genomic, transcriptional, and epigenetic information.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
DNA Methylation
Epigenomics / methods*
Gene Expression Regulation
Humans
Single-Cell Analysis / methods*

Abstract

Publication types

MeSH terms

Grants and funding