Abstract
Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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CRISPR-Cas Systems / drug effects*
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CRISPR-Cas Systems / radiation effects
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Cryptochromes / genetics
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DNA / genetics
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Gene Expression Regulation / genetics
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Gene Expression Regulation / radiation effects
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Humans
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Light
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Optogenetics
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Plasmids / genetics
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Protein Engineering
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RNA / biosynthesis
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RNA / genetics
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Streptococcus pyogenes / enzymology
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Streptococcus pyogenes / genetics
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Transcription, Genetic / drug effects
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Transcriptional Activation / drug effects*
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Transcriptional Activation / radiation effects*
Substances
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CRY2 protein, human
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Cryptochromes
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RNA
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DNA