Abstract
The fruit fly Drosophila melanogaster has been extensively used to identify genes required for photoreceptor cell function. We show that the ninaC gene, originally isolated as a Drosophila visual mutation with an electrophysiological phenotype, encodes two novel cytoskeletal proteins. We identified the DNA sequences encoding the ninaC gene by rescuing the electrophysiological phenotype using P-element-mediated germ line transformation. The ninaC locus is expressed as two extensively overlapping mRNAs encoding proteins of 1135 and 1501 amino acids. Both proteins contain a putative protein kinase domain joined to a domain homologous to the head region of the myosin heavy chain and are spatially restricted to photoreceptor cells.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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DNA / genetics
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DNA / isolation & purification
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DNA, Recombinant
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Drosophila melanogaster / genetics*
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Drosophila melanogaster / growth & development
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Electrophoresis, Polyacrylamide Gel
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Exons
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Fluorescent Antibody Technique
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Immune Sera
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Immunoassay
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Introns
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Molecular Sequence Data
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Mutation
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Myosins / genetics*
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Nucleic Acid Hybridization
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Phenotype
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Photoreceptor Cells / metabolism*
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Protein Kinases / genetics*
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RNA / genetics
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RNA, Messenger / genetics
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Recombinant Fusion Proteins / immunology
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Sequence Homology, Nucleic Acid
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Transformation, Genetic
Substances
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DNA, Recombinant
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Immune Sera
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RNA, Messenger
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Recombinant Fusion Proteins
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RNA
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DNA
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Protein Kinases
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Myosins
Associated data
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GENBANK/J03131
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GENBANK/M20230
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GENBANK/M20231