Abstract
Metabolism is predicted to generate formaldehyde, a toxic, simple, reactive aldehyde that can damage DNA. Here we report a synthetic lethal interaction in avian cells between ADH5, encoding the main formaldehyde-detoxifying enzyme, and the Fanconi anemia (FA) DNA-repair pathway. These results define a fundamental role for the combined action of formaldehyde catabolism and DNA cross-link repair in vertebrate cell survival.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldehyde Oxidoreductases / genetics
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Animals
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Cell Line
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Chickens / genetics
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DNA Repair*
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Fanconi Anemia / genetics
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Fanconi Anemia / metabolism*
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Fanconi Anemia Complementation Group C Protein / genetics
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Fanconi Anemia Complementation Group C Protein / physiology
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Fanconi Anemia Complementation Group L Protein / genetics
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Fanconi Anemia Complementation Group L Protein / physiology
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Formaldehyde / metabolism*
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Gene Knockout Techniques
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Metabolic Networks and Pathways
Substances
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Fanconi Anemia Complementation Group C Protein
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Formaldehyde
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Aldehyde Oxidoreductases
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formaldehyde dehydrogenase, glutathione-independent
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Fanconi Anemia Complementation Group L Protein