Abstract
The use of neural stem cells (NSCs) or their progeny oligodendrocyte precursor cells (OPCs) represents a promising repair strategy for many neurological disorders. However, the molecular events and biological features during the transition from NSCs to OPCs remain unclear. In the present study, we isolated NSCs from the embryonic rat forebrain and induced them into OPCs by using B104 conditioned medium (B104CM) in vitro. We then employed cDNA array technology to compare changes in gene expression between the two cell populations. Among 1,176 genes examined, 40 were differentially expressed, and some of them may be involved in OPC differentiation from NSCs. Our findings thus provide new insights into the molecular basis of differentiation of OPCs from NSCs.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Count
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Cell Differentiation / drug effects
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Cell Differentiation / genetics
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Cells, Cultured
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Culture Media, Conditioned / pharmacology
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Embryo, Mammalian
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Gene Expression Profiling
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Gene Expression* / drug effects
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Gene Expression* / physiology
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Glial Fibrillary Acidic Protein / metabolism
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Glycoproteins / metabolism
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Immunohistochemistry / methods
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Intermediate Filament Proteins / metabolism
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Nerve Tissue Proteins / metabolism
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Nestin
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Neurons / metabolism
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O Antigens / metabolism
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Oligodendroglia / metabolism*
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Oligonucleotide Array Sequence Analysis / methods
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Prosencephalon / cytology
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RNA, Messenger / biosynthesis
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Rats
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Rats, Wistar
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Receptor, Platelet-Derived Growth Factor alpha / metabolism
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Stem Cells / metabolism*
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Tubulin / metabolism
Substances
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Culture Media, Conditioned
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Glial Fibrillary Acidic Protein
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Glycoproteins
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Intermediate Filament Proteins
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Nerve Tissue Proteins
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Nes protein, rat
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Nestin
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O Antigens
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RNA, Messenger
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Tubulin
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Receptor, Platelet-Derived Growth Factor alpha