Abstract
To investigate the role of the LIM-kinase (LIMK) family in the regulation of ADF/cofilin phosphorylation and synaptic function in the mammalian central nervous system (CNS), we conducted biochemical and electrophysiological analysis using mice that were genetically altered in the expression of LIMK-1 and LIMK-2. We showed here that while LIMK-2 knockout mice exhibited minimal abnormalities, the LIMK-1/2 double knockout mice were more severely impaired in both ADF/cofilin phosphorylation and excitatory synaptic function in the CA1 region of the hippocampus. These results indicate a critical role for the LIMK family in the regulation of ADF/cofilin and synaptic function in the brain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actin Depolymerizing Factors
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Animals
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Cerebellum / physiology
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DNA-Binding Proteins / deficiency
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Destrin
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Hippocampus / physiology
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Kinetics
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Lim Kinases
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Mice
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Mice, Knockout
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Microfilament Proteins / metabolism*
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Phosphorylation
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Protein Kinases / deficiency
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Protein Kinases / genetics
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Protein Kinases / metabolism*
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Protein Serine-Threonine Kinases / deficiency
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Synapses / physiology*
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Zinc Fingers
Substances
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Actin Depolymerizing Factors
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DNA-Binding Proteins
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Destrin
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Dstn protein, mouse
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Microfilament Proteins
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Protein Kinases
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Lim Kinases
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Limk1 protein, mouse
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Limk2 protein, mouse
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Protein Serine-Threonine Kinases