Abstract
Y box-binding protein-1 (YB-1), a member of the DNA binding protein family, interacts with inverted CCAAT boxes (Y-boxes). Y-boxes are located on the promoter of numerous genes, such as DNA topoisomerase IIalpha (Topo IIalpha), proliferating cell nuclear antigen (PCNA) and multidrug resistance 1 (MDR1). In this study, we used immunohistochemical (IHC) staining to detect YB-1 expression in 59 lung cancer tissues and to evaluate whether YB-1 expression was associated with the expression of YB-1 target genes such as Topo IIalpha, PCNA and MDR1 in human lung carcinoma. Twenty-eight out of 59 cases (47.5%) were stained positive for YB-1 in the cytoplasm, while 30 out of 59 cases (50.8%) were positive for PCNA in the nuclei. Topo IIalpha-positive cells were detected in 16 out of 59 cases (27.1%). Eight out of 59 cases (13.6%) had greater than 5% P-gp positive cells expression. There was a significant correlation between the YB-1 and Topo IIalpha expression in small cell lung cancer (SCLC) (p=0.0242). YB-1 expression also correlated with PCNA expression in non-small cell lung cancer (NSCLC) (p=0.0001). Higher levels of YB-1 expression were associated with T3-4 and Stage III-IV tumors in adenocarcinomas (p=0.0072; p=0.0168). In contrast, no relationship was found between YB-1 expression and P-gp expression. Our study suggests that YB-1 expression correlates with Topo IIalpha and PCNA expression in lung cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
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Adenocarcinoma / enzymology
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism
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Adenocarcinoma / pathology
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Aged
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Antigens, Neoplasm
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CCAAT-Enhancer-Binding Proteins / biosynthesis*
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CCAAT-Enhancer-Binding Proteins / genetics
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Carcinoma, Small Cell / enzymology
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Carcinoma, Small Cell / genetics
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Carcinoma, Small Cell / metabolism
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Carcinoma, Small Cell / pathology
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Carcinoma, Squamous Cell / enzymology
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Carcinoma, Squamous Cell / metabolism
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DNA Topoisomerases, Type II / biosynthesis*
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DNA Topoisomerases, Type II / genetics
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DNA-Binding Proteins*
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Immunohistochemistry
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Lung Neoplasms / enzymology
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Lung Neoplasms / genetics
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Lung Neoplasms / metabolism*
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Lung Neoplasms / pathology
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Male
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NFI Transcription Factors
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Nuclear Proteins
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Proliferating Cell Nuclear Antigen / biosynthesis*
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Proliferating Cell Nuclear Antigen / genetics
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Transcription Factors*
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Tumor Cells, Cultured
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Y-Box-Binding Protein 1
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Antigens, Neoplasm
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CCAAT-Enhancer-Binding Proteins
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DNA-Binding Proteins
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NFI Transcription Factors
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Nuclear Proteins
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Proliferating Cell Nuclear Antigen
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Transcription Factors
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Y-Box-Binding Protein 1
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YBX1 protein, human
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DNA Topoisomerases, Type II