Abstract
We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05-0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aneuploidy*
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Chromosomes, Artificial, Bacterial / genetics
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Cloning, Molecular
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Female
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Gene Dosage*
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Genome, Human*
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Genomics / methods*
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Humans
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Male
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Oligonucleotide Array Sequence Analysis / methods*
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Polymerase Chain Reaction
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Polyploidy
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Tumor Cells, Cultured
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X Chromosome / genetics