Abstract
Here we report the genomic organization and mapping of the X-linked inhibitor of apoptosis gene (BIRC4, also known as XIAP and hILP) and the identification of a closely related transcript. BIRC4 is located on Xq25 and is composed of seven exons. The intron/exon structure is highly conserved between the mouse homologue and its human counterpart. Four bands cross-react with a BIRC4 coding region probe on a genomic Southern blot. One of these cross-reactive bands encodes an intronless gene that expresses a 2.2-kb transcript solely in the testis. This testis-specific transcript contains a putative open reading frame (ORF) that is homologous to the carboxy-terminal end of BIRC4; overexpression of this ORF shows protective effects against BAX-induced apoptosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis*
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Base Sequence
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Blotting, Southern
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Blotting, Western
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Cell Line
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Cloning, Molecular
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Conserved Sequence
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Exons
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Female
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Gene Library
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HeLa Cells
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Humans
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Introns
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Male
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Mice
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Molecular Sequence Data
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Open Reading Frames
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Proteins / analysis
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Proteins / genetics*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / physiology
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Proto-Oncogene Proteins c-bcl-2*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Testis / metabolism
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Transfection
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X Chromosome / genetics*
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X-Linked Inhibitor of Apoptosis Protein
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bcl-2-Associated X Protein
Substances
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BAX protein, human
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Bax protein, mouse
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Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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RNA, Messenger
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X-Linked Inhibitor of Apoptosis Protein
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XIAP protein, human
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bcl-2-Associated X Protein