Modulation of WHSC2 expression in human endothelial cells

FEBS Lett. 2000 Dec 29;487(2):166-70. doi: 10.1016/s0014-5793(00)02335-8.

Abstract

WHSC2, a novel gene recently isolated within the critical region of Wolf-Hirschhorn syndrome, is expressed in endothelial cells. WHSC2 is downregulated by HIV-1 Tat, whereas it is not modulated by angiogenic and pro-differentiative molecules. WHSC2 encodes a basic polypeptide of 528 amino acids. The in vitro translated protein shows a molecular weight of 57 kDa. WHSC2 has two nuclear translocation sequences which actively mediate its transport to the nucleus, as shown in whsc2-GFP-transfected NIH-3T3. We also found a helix-loop-helix (HLH) motif in region 130-185. Since members of the HLH family control differentiation and cell cycle progression, we hypothesize that WHSC2 may function as a transcriptional repressor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Cell Cycle
  • Cell Differentiation
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • Endothelial Growth Factors / pharmacology
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / physiology*
  • Fibroblast Growth Factor 2 / pharmacology
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology*
  • Gene Products, tat / pharmacology
  • Genes, Reporter
  • Green Fluorescent Proteins
  • Growth Substances / pharmacology*
  • HIV-1
  • Helix-Loop-Helix Motifs
  • Hepatocyte Growth Factor / pharmacology
  • Humans
  • Luminescent Proteins / genetics
  • Lymphokines / pharmacology
  • Mice
  • Molecular Sequence Data
  • Protein Biosynthesis
  • Protein Structure, Secondary
  • Protein Transport
  • Proteins / chemistry
  • Proteins / genetics*
  • Proteins / metabolism*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
  • Transcriptional Elongation Factors
  • Transfection
  • Umbilical Veins
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • tat Gene Products, Human Immunodeficiency Virus

Substances

  • Endothelial Growth Factors
  • Gene Products, tat
  • Growth Substances
  • Luminescent Proteins
  • Lymphokines
  • NELFA protein, human
  • Proteins
  • Recombinant Proteins
  • Transcriptional Elongation Factors
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • tat Gene Products, Human Immunodeficiency Virus
  • Fibroblast Growth Factor 2
  • Green Fluorescent Proteins
  • Hepatocyte Growth Factor