Abstract
Peroxisome biogenesis disorders (PBDs) contain various clinical phenotypes; Zellweger syndrome (ZS), neonatal adrenoleukodystrophy (NALD), and infantile Refsum disease (IRD), decreasing in the clinical severity in this order. We found that all IRD cell lines and some NALD lines belonging to several different complementation groups are temperature-sensitive in peroxisome assembly; that is, they lacked catalase-positive peroxisomes at 37°C, but do gain the peroxisomes at 30°C. We identified heterozygous mutations E55K/R119Stop in the PEX2 gene of an IRD patient of complementation group F. The E55K mutation was the direct cause of the temperature-sensitivity because similar phenotypes could be transferred to PEX2-defective CHO cells by transfecting the mutant gene. Thus, temperature-sensitive peroxisome assembly is representative of milder forms of PBDs.
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The main part of this study was published by Imamura et al. (1).
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Osumi, T., Imamura, A., Tsukamoto, T. et al. Temperature sensitivity in peroxisome assembly processes characterizes milder forms of peroxisome biogenesis disorders. Cell Biochem Biophys 32, 165–170 (2000). https://doi.org/10.1385/CBB:32:1-3:165
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DOI: https://doi.org/10.1385/CBB:32:1-3:165