Rapid PublicationsA calcium-activated chloride channel (HCLCA1) is strongly related to IL-9 expression and mucus production in bronchial epithelium of patients with asthma☆
Section snippets
Patient profile
Nine asthmatic subjects (mean age, 39 ± 12 years) and 10 healthy nonatopic control subjects (mean age, 40 ± 13 years) were recruited. Asthma severity was assessed on the basis of the Aas scoring system.16 Subjects were excluded if they had a lower respiratory tract infection, an exacerbation of their asthma for up to 8 weeks before the study, or treatment with corticosteroids within 4 months before the study. All subjects were nonsmokers. Approval was obtained from the local ethics committee,
Baseline description of asthmatic subjects compared with healthy control subjects
Asthmatic subjects had significantly increased numbers of major basic protein-immunoreactive eosinophils (23.8 ± 18.4/mm2 of submucosa vs 4.0 ± 4.8/mm2 of submucosa, P < 0.01) and significantly increased number of CD3-immunoreactive T cells (83.7 ± 33.7/mm2 of submucosa vs 51.5 ± 19.3/mm2 of submucosa, P < .05) in the submucosa compared with that seen in normal control subjects. IL-9 immunoreactivity was significantly higher in asthmatic subjects compared with that in healthy control subjects
Discussion
This is the first study that demonstrates the significantly increased expression of HCLCA1 in asthmatic subjects and suggests that this channel is involved in regulating IL-9-dependent mucus gene expressions in asthma. Although the phenomenon of mucus hypersecretion and inflammation have been documented as features of asthma for many years, little is known about their potential relationship. A number of cytokines, including IL-4, IL-13, and IL-9, have been shown, in human asthmatic subjects and
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Reprint requests: Qutayba Hamid, MD, PhD, Meakins-Christie Laboratories, 3626 St Urbain St, Montreal, Quebec H2X 2P2, Canada.