Abstract
Here, we describe a quick and easy version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method can be performed when only a few transformants are needed. The procedure can take less than an hour, depending on the duration of the heat shock. It can be used to transform yeast cells from various stages of growth and storage. Cells can be transformed from freshly grown cells as well as cells stored on a plate at room temperature or in a refrigerator.
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Change history
20 November 2008
In the version of this article initially published, in the Reagent Setup section on p. 36, the recipe for LiAc (1.0 M) called for 102 g of LiAc dihydrate. This should be 10.2 g. The error has been corrected in the HTML and PDF versions of the article.
References
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Gietz, R., Schiestl, R. Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2, 35–37 (2007). https://doi.org/10.1038/nprot.2007.14
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DOI: https://doi.org/10.1038/nprot.2007.14
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