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Genomic scanning for expressed sequences in Xp21 identifies the glycerol kinase gene

Nature Genetics volume 4, pages 367–372 (1993)Cite this article

Abstract

Rapid genomic scanning methods are required to identify expressed sequences and we report an efficient, sensitive and specific approach which relies upon hybridization of an amplified, labeled cDNA library to digested cosmid DNA. We identified expressed sequences within a cosmid in the glycerol kinase (GK) “critical region” of Xp21 that had impressive similarity to prokaryotic GKs. We used this genomic sequence information to clone the human hepatic GK cDNA. Independent confirmation of the identity of this gene was obtained by functional complementation of GK deficient E. coli mutants with a construct containing the complete human X–linked GK coding sequence.

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Authors and Affiliations

  1. Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas, 77030, USA

    Weiwen Guo, Kim Worley, Volker Adams, Desirée Sylvester-Jackson, Yao-Hua Zhang, Donna D. Fogt, Stella Madu & Edward R.B. McCabe

  2. Department of Pediatrics, Baylor College of Medicine, Houston, Texas, 77030, USA

    Jestina Mason, Desirée Sylvester-Jackson, Jeffrey A. Towbin & Edward R.B. McCabe

  3. Department of Cell Biology, Baylor College of Medicine, Houston, Texas, 77030, USA

    David A. Wheeler

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  1. Weiwen Guo
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Guo, W., Worley, K., Adams, V. et al. Genomic scanning for expressed sequences in Xp21 identifies the glycerol kinase gene. Nat Genet 4, 367–372 (1993). https://doi.org/10.1038/ng0893-367

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