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A new bacteriophage P1–derived vector for the propagation of large human DNA fragments

Nature Genetics volume 6, pages 84–89 (1994)Cite this article

Abstract

We have designed a P1 vector (pCYPAC–1) for the introduction of recombinant DNA into E. coli using electroporation procedures. The new cloning system, P1–derived arteficial chromosomes (PACs), was used to establish an initial 15,000 clone library with an average insert size of 130–150 kilobase pairs (kb). No chimaerism has been observed in 34 clones, by fluorescence in situ hybridization. Similarly, no insert instability has been observed after extended culturing, for 20 clones. We conclude that the PAC cloning system will be useful in the mapping and detailed analysis of complex genomes.

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Author information

Author notes

  1. Panayiotis A. loannou

    Present address: The Cyprus Institute of Neurology and Genetics, P.O. Box 3462, Nicosia, Cyprus

  2. Chris T. Amemiya

    Present address: Center for Human Genetics, Boston University School of Medicine, 80 East Concord St, Boston, Massachusetts, 02118, USA

  3. Peter M. Kroisel

    Present address: Department of Medical Biology and Human Genetics, Harrachgasse 21/8, A-8010, Graz, Austria

Authors and Affiliations

  1. Human Genome Center L-452, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, California, 94551, USA

    Panayiotis A. loannou, Chris T. Amemiya, Jeffrey Garnes, Peter M. Kroisel, Chira Chen, Mark A. Batzer & Pieter J. de Jong

  2. Division of Biology, 147-75, California Institute of Technology, Pasadena, California, 91125, USA

    Hiroaki Shizuya

  3. Human Genetics Department, Roswell Park Cancer Institute, Elm & Carlton Streets, Buffalo, New York, 14263, USA

    Chira Chen & Pieter J. de Jong

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  1. Panayiotis A. loannou
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loannou, P., Amemiya, C., Garnes, J. et al. A new bacteriophage P1–derived vector for the propagation of large human DNA fragments. Nat Genet 6, 84–89 (1994). https://doi.org/10.1038/ng0194-84

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  • DOI: https://doi.org/10.1038/ng0194-84

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