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Targeted gene disruption in somatic zebrafish cells using engineered TALENs

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Figure 1: Target sequences, frequencies of mutations and mutations induced by TALENs in embryonic zebrafish cells.

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Acknowledgements

We thank D. Voytas and A. Bogdanove for helpful discussions in the early stages of this project and J. Foley, M. Maeder, S. Thibodeau-Beganny, F. Zhang, M. Christian and D. Voytas for help with characterizing the hey2- and gria3a-targeted ZFN pairs. This work was supported by US National Institutes of Health (NIH) R01 GM088040 (J.K.J. & R.T.P.), NIH Director's Pioneer Award DP1 OD006862 (J.K.J.), NIH T32 CA009216 (J.D.S.), NIH K01 AG031300 (J.-R.J.Y.), the Jim and Ann Orr Massachusetts General Hospital (MGH) Research Scholar award (J.K.J.), and the Charles and Ann Sanders MGH Research Scholar award (R.T.P.).

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Correspondence to J Keith Joung or Jing-Ruey J Yeh.

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Supplementary Figures 1–12, Supplementary Table 1 and Supplementary Methods (PDF 1457 kb)

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Sander, J., Cade, L., Khayter, C. et al. Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nat Biotechnol 29, 697–698 (2011). https://doi.org/10.1038/nbt.1934

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