Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Isolation of a cDNA encoding the vascular type-1 angiotensin II receptor

Nature volume 351, pages 233–236 (1991)Cite this article

Abstract

ANGIOTENSIN II is an important effector molecule controlling blood pressure and volume in the cardiovascular system1. Its importance is manifested by the efficacy of angiotensin-converting enzyme inhibitors in the treatment of hypertension and congestive heart failure2,3. Angiotensin II interacts with two pharmacologi-cally distinct subtypes of cell-surface receptors, AT1 and AT2 (ref. 4). AT1 receptors seem to mediate the major cardiovascular effects of angiotensin II5,6. Here we report the isolation by expression cloning of a complementary DNA encoding a unique protein with the pharmacological specificity of a vascular AT1 receptor. Hydropathic modelling of the deduced protein suggests that it shares the seven-transmembrane-region motif with the G protein-coupled receptor superfamily7. Knowledge of the AT1 receptor primary sequence should now permit structural analysis, definition of the angiotensin II receptor gene family and delineation of the contribution of AT receptors to the genetic component of hypertension.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Peach, M. J. Physiol. Rev. 57, 313–370 (1977).

    Article  CAS  Google Scholar 

  2. Bauer, J. H. Am. J. Med. 77 (Suppl. 2A), 43–51 (1984).

    Article  CAS  Google Scholar 

  3. Davis, R., Ribner, H. S., Keung, E., Sonnenblick, E. H. & LeJemtel, T. H. New Engl. J. Med. 301, 117–121 (1979).

    Article  CAS  Google Scholar 

  4. Bumpus, F. M. et al. Hypertension (in the press).

  5. Wong, P. C. et al. J. Pharmac. exp. Ther. 255, 584–592 (1990).

    CAS  Google Scholar 

  6. Dudley, D. T. et al. Molec. Pharmac. 38, 370–377 (1990).

    CAS  Google Scholar 

  7. Dohlman, H. G., Caron, M. C. & Lefkowitz, R. J. Biochemistry 26, 2657–2664 (1987).

    Article  CAS  Google Scholar 

  8. Wong, G. C. et al. Science 228, 810–815 (1985).

    Article  ADS  CAS  Google Scholar 

  9. Seed, B. & Aruffo, A. Proc. natn. Acad. Sci. U.S.A. 84, 3365–3369 (1987).

    Article  ADS  CAS  Google Scholar 

  10. Socorro, L., Alexander, R. W. & Griendling, K. K. Biochem. J. 265, 799–807 (1990).

    Article  CAS  Google Scholar 

  11. Douglas, J. G. Am. J. Physiol. 253, F1–F7 (1987).

    CAS  PubMed  Google Scholar 

  12. Chiu, A. T. et al. J. Pharmac. exp. Ther. 250, 867–874 (1989).

    CAS  Google Scholar 

  13. Chang, R. S. L. & Lotti, V. J. Molec. Pharmac. 29, 347–351 (1990).

    Google Scholar 

  14. Chiu, A. T. et al. Eur. J. Pharmac. 170, 117–118 (1989).

    Article  CAS  Google Scholar 

  15. Griendling, K. K. et al. J. biol. Chem. 261, 5901–5906 (1986).

    CAS  PubMed  Google Scholar 

  16. Kozak, M. Cell 44, 283–292 (1986).

    Article  CAS  Google Scholar 

  17. Escher, E. Pharmac. Ther. 37, 37–55 (1988).

    Article  CAS  Google Scholar 

  18. Karnik, S. S., Sakmar, T. P., Chen, H.-A. & Khorana, G. Proc. natn. Acad. Sci. U.S.A. 85, 8459–8463 (1988).

    Article  ADS  CAS  Google Scholar 

  19. McFarland, K. C. et al. Science 245, 494–499 (1989).

    Article  ADS  CAS  Google Scholar 

  20. Yokota, Y. et al. J. biol. Chem. 264, 17649–17652 (1989).

    CAS  PubMed  Google Scholar 

  21. Arai, H., Hori, S., Aramori, I., Ohkubo, H. & Nakanishi, S. Nature 348, 730–732 (1990).

    Article  ADS  CAS  Google Scholar 

  22. Sakurai, T. et al. Nature 348, 732–735 (1990).

    Article  ADS  CAS  Google Scholar 

  23. Kemp, B. E. & Pearson, R. B. Trends Biochem. 15, 342–346 (1990).

    Article  CAS  Google Scholar 

  24. Capponi, A. M., Aguilera, G., Fakunding, J. L. & Catt, K. J. in Biochemical Regulation in Blood Pressure (ed. Soffer, R. L.) 205–262 (Wiley, New York, 1981).

    Google Scholar 

  25. Jackson, T. R., Blair, L. A. C., Marshall, J., Goedert, M. & Hanley, M. R. Nature 335, 437–440 (1988).

    Article  ADS  CAS  Google Scholar 

  26. Bouscarel, B., Blackmore, P. F. & Exton, J. H. J. biol. Chem. 263, 14913–14919 (1988).

    CAS  PubMed  Google Scholar 

  27. Phillips, M. I. A. Rev. Physiol. 49, 413–435 (1987).

    Article  CAS  Google Scholar 

  28. Millan, N. A. et al. Science 244, 1340–1342 (1989).

    Article  ADS  CAS  Google Scholar 

  29. Murphy, T. J. & Bylund, D. B. J. Pharmac. exp. Ther. 244, 571–578 (1988).

    CAS  Google Scholar 

  30. Chen, C. A. & Okayama, H. Biotechniques 6, 632–638 (1988).

    CAS  PubMed  Google Scholar 

  31. Alexander, R. W., Brock, T. A., Gimbrone, M. A. & Rittenhouse, S. E. Hypertension 7, 447–451 (1985).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, Department of Medicine,

    T. J. Murphy & Kenneth E. Bernstein

  2. Emory University School of Medicine, Atlanta, Georgia, 30322, USA

    T. J. Murphy & Kenneth E. Bernstein

  3. Division of Cardiology, Department of Medicine,

    T. J. Murphy, R. Wayne Alexander, Kathy K. Griendling & Marschall S. Runge

  4. Emory University School of Medicine, Atlanta, Georgia, 30322, USA

    T. J. Murphy, R. Wayne Alexander, Kathy K. Griendling & Marschall S. Runge

Authors
  1. T. J. Murphy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Wayne Alexander
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kathy K. Griendling
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marschall S. Runge
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kenneth E. Bernstein
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Murphy, T., Alexander, R., Griendling, K. et al. Isolation of a cDNA encoding the vascular type-1 angiotensin II receptor. Nature 351, 233–236 (1991). https://doi.org/10.1038/351233a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/351233a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing