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Construction of a functional human suppressor tRNA gene: an approach to gene therapy for β-thalassaemia

Nature volume 296, pages 537–540 (1982)Cite this article

Abstract

A human tRNALys gene was converted to an amber suppressor by site-specific mutagenesis of the anticodon. The mutated tRNALys gene directed synthesis of a tRNA that suppressed the UAG amber nonsense mutation in β0 thalassaemia mRNA. Such genes may be used to detect other nonsense mutations in mammalian cells and may provide an approach to gene therapy for β0 thalassaemia due to nonsense mutations.

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Author information

Author notes

  1. Gary F. Temple

    Present address: Bethesda Research Laboratories Gaithersburg, Maryland, USA

Authors and Affiliations

  1. Howard Hughes Medical Institute Laboratory, and Departments of Medicine, Biochemistry and Biophysics, University of California, San Francisco, California, 94143, USA

    Gary F. Temple, Andrée M. Dozy & Yuet Wai Kan

  2. Department of Microbiology, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada

    Kenneth L. Roy

Authors
  1. Gary F. Temple
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  2. Andrée M. Dozy
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  3. Kenneth L. Roy
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  4. Yuet Wai Kan
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Temple, G., Dozy, A., Roy, K. et al. Construction of a functional human suppressor tRNA gene: an approach to gene therapy for β-thalassaemia. Nature 296, 537–540 (1982). https://doi.org/10.1038/296537a0

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