Skip to main content
Log in

Analysis of X-ray-induced aberrations in human chromosome 5 using high-resolution multicolour banding FISH (mBAND)

  • Published:
Chromosome Research Aims and scope Submit manuscript

Abstract

Peripheral lymphocytes were exposed to 4 Gy X-rays and aberrations were analysed in human chromosome 5 using high-resolution multicolour banding fluorescence in-situ hybridization (mBAND). This method is suited to detect simple and complex aberrations including peri- and paracentric inversions and exchanges between both chromosomes 5. Additionally, breakpoints can be assigned to specific regions in chromosome 5. Quantitative relationships of induced aberration types are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barrios L, Mior R, Caballin MR et al. (1989) Cytogenetic effects of radiotherapy breakpoint distribution in induced chromosome aberrations. Cancer Genet Cytogenet 41: 61–70.

    Google Scholar 

  • Bauchinger M, Schmid E, Zitzelsberger H, Braselmann H, Nahrstedt U (1993) Radiation-induced chromosome aberrations analysed by two-colour fluorescence in situ hybridization with composite whole chromosome-specific DNA probes and a pan-centromeric DNA probe. Int J Radiat Biol 64: 179–184.

    Google Scholar 

  • Chudoba I, Rubtsov N, Senger G, Junker K, Bleck C, Claussen U (1996) Improved detection of chromosome 16 rearrangements in acute myeloid leukemias using 16p and 16q specific microdissection libraries. Oncol Rep 3: 829–832.

    Google Scholar 

  • Chudoba I, Plesch A, Lörch T, Lemke J, Claussen U, Senger G (1999) High resolution multicolor-banding: a new technique for refined FISH analysis of humam chromosomes. Cytogenet Cell Genet 84: 156–160.

    Google Scholar 

  • Folle GA, Obe G (1995) Localization of chromosome breakpoints induced by AluI and BamHI in Chinese hamster ovary (CHO) cells treated in the G1 phase of the cell cycle. Int J Radiat Biol 68: 437–445.

    Google Scholar 

  • Folle GA, Obe G (1996) Intrachromosomal localization of breakpoints induced by the restriction endonucleases AluI and BamHI in Chinese hamster ovary cells treated in S-phase of the cell cycle. Int J Radiat Biol 69: 447–457.

    Google Scholar 

  • Folle GA, Boccardo E, Obe G (1997) Localization of chromosome breakpoints induced by DNaseI in Chinese hamster ovary (CHO) cells. Chromosoma 106: 391–399.

    Google Scholar 

  • Folle GA, Martinzes-López W, Boccardo E, Obe G (1998) Localization of chromosome breakpoints: implication of the chromatin structure and nuclear architecture. Mutation Res 404: 17–26.

    Google Scholar 

  • Gray JW, Pinkel D, Brown JM (1994) Review. Fluorescence in situ hybridization in cancer and radiation biology. Radiat Res 137: 275–289.

    Google Scholar 

  • Holmberg M, Jonasson J (1973) Preferential location of X-ray induced chromosome breakage in the R-bands of human chromosomes. Hereditas 74: 57–67.

    Google Scholar 

  • Holmquist GP (1992) Review article: Chromosome bands, their chromatin flavors, and their functional features. Am J Hum Genet 51: 17–37.

    Google Scholar 

  • Johnson RL, Brenner DJ, Nath J, Tucker JD, Geard CR (1999) Review. Radiation-induced breakpoint misrejoining in human chromosomes: random or non-random. Int J Radiat Biol 75: 131–141.

    Google Scholar 

  • Knehr S, Zitzelsberger H, Braselmann H, Bauchinger M (1994) Analysis for DNA-proportional distribution of radiation-induced chromosome aberrations in various triple combinations of human chromosomes using fluorescence in situ hybridization. Int J Radiat Biol 65: 683–690.

    Google Scholar 

  • Knehr S, Huber R, Braselmann H, Schraube H, Bauchinger M (1999) Multicolour FISH painting for the analysis of chromosomal aberrations induced by 220 kV X-rays and fission neutrons. Int J Radit Biol 75: 407–418.

    Google Scholar 

  • Kreth G, Münkel Ch, Langowski J, Cremer T, Cremer C (1998) Chromatin structure and chromosome aberrations: modelling of damage induced by isotropic and localized irradiation. Mutation Res 404: 77–88.

    Google Scholar 

  • Lucas JN, Tenjin T, Straume T et al. (1989) Rapid human chromosome aberration analysis using fluorescence in situ hybridization. Int J Radiat Biol 56: 35–44.

    Google Scholar 

  • Lucas JN, Hill FS, Chen AM, Burk CE (1997) A rapid method for measuring pericentric inversions using fluorescence in situ hybridization (FISH). Int J Radiat Biol 71: 29–33.

    Google Scholar 

  • Martinez-López W, Boccardo EM, Folle GA, Porro V, Obe G (1998) Intrachromosomal localization of aberration breakpoints induced by neutrons and gamma rays in Chinese hamster ovary cells. Radiat Res 150: 585–592.

    Google Scholar 

  • McKinlay Gardner RJ, Sutherland GR (1996) Chromosome Abnormalities and Genetic Counselling. Oxford: Oxford University Press, pp 137–158.

    Google Scholar 

  • Morton NE (1991) Parameters of the human genome. Proc Natl Acad Sci 88: 7474–7476.

    Google Scholar 

  • Nakagome Y, Matsubara T, Fujita H (1983) Distribution of breakpoints in human structural rearrangements. Am J Human Genet 35: 288–300.

    Google Scholar 

  • Natarajan AT, Vyas RC, Darroudi F, Vermeulen S (1992) Frequencies of X-ray-induced chromosome translocations in human peripheral lymphocytes as detected by in situ hybridization using chromosome-specific DNA libraries. Int J Radiat Biol 61: 199–203.

    Google Scholar 

  • Natarajan AT, Balajee AS, Boei JJWA et al. (1994) Recent developments in the assessment of chromosomal damage. Int J Radiat Biol 66: 615–623.

    Google Scholar 

  • Natarajan AT, Boei JJWA, Vermeulen S, Balajee AS (1996) Frequencies of X-ray induced pericentric inversions and centric rings in human lymphocytes detected by FISH using chromosome arm specific DNA libraries. Mutation Res 372: 1–7.

    Google Scholar 

  • Neubauer S, Dunst J, Gebhart E (1997) The impact of complex chromosomal rearrangements on the detection of radiosensitivity in cancer patients. Radiother Oncol 43: 189–195.

    Google Scholar 

  • Sachs RK, Chen AM, Brenner DJ (1997a) Review: Proximity effects in the production of chromosome aberrations by ionizing radiation. Int J Radiat Biol 71: 1–19.

    Google Scholar 

  • Sachs RK, Brenner DJ, Chen AM, Hahnfeldt P, Hlatky LR (1997b) Intra-arm and interarm chromosome intrachanges: Tools for probing the geometry and dynamics of chromatin. Radiat Res 148: 330–340.

    Google Scholar 

  • Savage JRK (1995) The transmission of FISH-painted patterns derived from complex chromosome exchanges. Mutation Res 347: 87–95.

    Google Scholar 

  • Savage JRK (1997) A note on inter-arm intrachange patterns resulting from dual-arm fish painting. Mutation Res 373: 265–269.

    Google Scholar 

  • Savage JRK, Simpson PJ (1994a) FISH “painting” patterns resulting from complex exchanges. Mutation Res 312: 51–60.

    Google Scholar 

  • Savage JRK, Simpson P (1994b) On the scoring of FISH-“painted” chromosome-type exchange aberrations. Mutation Res 307: 345–353.

    Google Scholar 

  • Savage JRK, Tucker JD (1996) Nomenclature systems for FISH-painted chromosome aberrations. Mutation Res 366: 153–161.

    Google Scholar 

  • Schmid E, Zitzelsberger H, Braselmann H, Gray JW, Bauchinger M (1992) Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes. Int J Radiat Biol 62: 673–678.

    Google Scholar 

  • Senger G, Ragoussis J, Trowsdale J, Sheer D (1993) Fine mapping of human MHC class II region within chromosome 6p21 and evaluation of probe ordering using fluorescence in situ hybridization. Cytogenet Cell Genet 64: 49–53.

    Google Scholar 

  • Simpson PJ, Savage JRK (1994) Identification of X-ray-induced complex chromosome exchanges using fluorescence in situ hybridization: a comparison at two doses. Int J Radiat Biol 66: 629–632.

    Google Scholar 

  • Simpson PJ, Savage JRK (1995a) Detecting “hidden” exchange events within X-ray-induced aberrations using multicolour chromosome paints. Chromosome Res 3: 69–72.

    Google Scholar 

  • Simpson PJ, Savage JRK (1995b) Estimating the true frequency of X-ray-induced complex chromosome exchanges using fluorescence in situ hybridization. Int J Radiat Biol 67: 37–45.

    Google Scholar 

  • Simpson PJ, Papworth DG, Savage JRK (1999) X-ray-induced simple, pseudosimple and complex exchanges involving two distinctly painted chromosomes. Int J Radiat Biol 75: 11–18.

    Google Scholar 

  • Speicher MR, Ballard SG, Ward DS (1996) Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nature Genet 12: 368–375.

    Google Scholar 

  • Tucker JD, Senft JR (1994) Analysis of naturally occurring and radiation-induced breakpoint locations in human chromosomes 1, 2 and 4. Radiat Res 140: 31–36.

    Google Scholar 

  • Tucker JD, Morgan WF, Awa AA et al. (1994) A proposed system for scoring structural aberrations detected by chromosome painting. Cytogenet Cell Genet 68: 211–221.

    Google Scholar 

  • Wojcik A, Streffer C (1998) Comparison of radiation-induced aberration frequencies in chromosomes 1 and 2 of two human donors. Int J Radiat Biol 74: 573–581.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Johannes, C., Chudoba, I. & Obe, G. Analysis of X-ray-induced aberrations in human chromosome 5 using high-resolution multicolour banding FISH (mBAND). Chromosome Res 7, 625–633 (1999). https://doi.org/10.1023/A:1009284018942

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1009284018942

Navigation