Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Volume 1823, Issue 4, April 2012, Pages 930-939
Roles of SAM and DDHD domains in mammalian intracellular phospholipase A1 KIAA0725p☆
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Highlights
► KIAA0725p and p125 are novel phosphoinositides-binding proteins. ► The SAM-DDHD domain in KIAA0725p is a minimal region for PI(4)P-binding. ► A positive charge cluster in the SAM domain is responsible for PI(4)P-binding. ► The lipid binding is necessary for targeting of KIAA0725p to Golgi/ERGIC membranes. ► The DDHD domain in KIAA0725p and PA-PLA1 is essential for their PLA1 activities.
Abbreviations
DOPA
1,2-dioleoyl-sn-phosphatidic acid
ER
endoplasmic reticulum
ERGIC
endoplasmic reticulum-Golgi intermediate compartment
GST
glutathione-S-transferase
iPLA1
intracellular phospholipase A1
LPA
1-oleoyl-lysophosphatidic acid
LUV
large unilamellar vesicles
PA
phosphatidic acid
PC
phosphatidylcholine
PE
phosphatidylethanolamine
PH
pleckstrin homology
PI(3)P
phosphatidylinositol 3-phosphate
PI(4)P
phosphatidylinositol 4-phosphate
PI(4,5)P2
phosphatidylinositol 4,5-bisphosphate
PI(5)P
phosphatidylinositol 5-phosphate
PI
phosphatidylinositol
PIP
phosphatidylinositol phosphate
PLA1
phospholipase A1
PS
phosphatidylserine
SAM
sterile alpha-motif
TLC
thin-layer chromatography
WT
wild-type
Keywords
KIAA0725p
Phospholipase A1
Phosphoinositide
Phosphatidylinositol 4-phosphate
SAM domain
DDHD domain
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This work was supported in part by Grants-in-Aid for Scientific Research 23570174 to H.I. and 20570190 to K.T. from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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These authors contributed equally to this work.
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