Original contributionIL-10 and TNF-α polymorphisms and the recovery from HCV infection
Section snippets
Abbreviations
- EIA
enzyme immunoassay
- HCV
hepatitis C virus
- HLA
human leukocyte antigens
- IFN
interferon
- IL
interleukin
- RIBA
reverse immunoblotting assay
- SNP
single nucleotide polymorphism
- TNF
tumor necrosis factor
Patients
Eighteen Sicilian blood donors (age, 45.0 ± 1.3; 12 men and 6 women) with anti-HCV positivity without chronic infection gave informed consent and entered the study. These patients had spontaneously recovered from previous HCV infection as demonstrated by anti-HCV positive with persistently normal aminotransferases and negative HCV-RNA on three different tests 4 months apart. Data from this group were compared with those from 42 Sicilian patients (age, 45.3 ±3.2 years; 20 men and 22 women) with
Results
The clean effect of a given gene is a function of the gene pair combination received from parents and for both TNF-α and IL-10 SNPs a gene dosage effect on cytokine production has been claimed 8, 28. Thus, we have evaluated the frequencies of the different genotypes in the subjects with spontaneous recovery from HCV infection, in patients with chronic HCV infection and in healthy patients.
As reported in Table 1, a significant different distribution (p = 0.002) of -863C→A TNF-α genotypes was
Discussion
This study demonstrates that TNF-α gene SNP -863C→A and IL-10 gene SNP -1082G→A were significantly associated to a spontaneous clearance of HCV in Sicilians. TNF-α and IL-10 have predominantly opposing roles in the inflammatory responses. TNF-α has a wide range of proinflammatory activities, determining strength, effectiveness, and duration of local and systemic inflammatory reactions [30]. The type 2 cytokine IL-10, besides promoting humoral immune responses, limits and terminates inflammatory
Acknowledgements
These studies have been supported by grants from Ministry of Education, University and Research, Rome, ex 60% to D.L., G.C.R., G.C., and C.C.
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