Crystal structure of tandem type III fiibronectin domains from drosophila neuroglian at 2.0 å

doi:10.1016/0896-6273(94)90326-3

Neuron

Volume 12, Issue 4, April 1994, Pages 717-731

https://doi.org/10.1016/0896-6273(94)90326-3 Get rights and content

Abstract

We report the crystal structure of two adjacent fibronectin type III repeats from the Drosophila neural cell adhesion molecule neuroglian. Each domain consists of two antiparallel 0 sheets and is folded topologically identically to single fibronectin type III domains from the extracellular matrix proteins tenascin and fibronectin. β bulges and left-handed polyproline II helices disrupt the regular β sheet structure of both neuroglian domains. The hydrophobic interdomain interface includes a metalbinding site, presumably involved in stabilizing the relative orientation between domains and predicted by sequence comparision to be present in the vertebrate homolog molecule L1. The neuroglian domains are related by a near perfect 2-fold screw axis along the longest molecular dimension. Using this relationship, a model for arrays of tandem fibronectin type III repeats in neuroglian and other molecules is proposed.

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Citation Excerpt :
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Neuron

ArticleCrystal structure of tandem type III fiibronectin domains from drosophila neuroglian at 2.0 å

Abstract

J. Mo. Biol.

J. Biol. Chem.

J. Mol. Biol.

Cell

Anal. Biochem.

J. Mol. Graphics

Adv. Prot. Chem.

Cell

J. Mol. Biol.

Cell

Cell

Meth. Enzymol.

J. Biol. Chem.

Neurosci. Res.

Several extracellular domains of the neural cell adhesion molecule L1 are involved in neurite outgrowth and cell body adhesion

J. Neurosci.

1H NMR assignment and secondary structure of the cell adhesion type III module of fibronectin

Biochemistry

Structural design and molecular evolution of a cytokine receptor superfamily

Proposed acquisition of an animal protein domain by bacteria

Crystal structure of domains 3 and 4 of rat CD4: relation to the NHZ terminal domains

Science

Free R value: a novel statistical quantity for assessing the accuracy of crystal structures

Nature

Characterization and use of the Drosophila metallothionein promoter in cultured Drosophila melanogaster cells

Nucl. Acids Res.

Cloning tissue culture cells

Human Growth Hormone and Extracellular Domain of Its Receptor: Crystal Structure of the Complex

Science

Of URFS and ORFS: A Primer on How to Analyze Derived Amino Acid Sequences

Different extracellular domains of the neural cell adhesion molecule (N-CAM) are involved in different functions

J. Cell Biol.

Molecular genetics of neuronal recognition in Drosophila: evolution and function of the immunoglobulin superfamily cell adhesion molecules

Article
Crystal structure of tandem type III fiibronectin domains from drosophila neuroglian at 2.0 å

¹H NMR assignment and secondary structure of the cell adhesion type III module of fibronectin