High-density cDNA filter analysis of the expression profiles of the genes preferentially expressed in human brain

doi:10.1016/0378-1119(95)00396-N

Gene

Volume 164, Issue 2, 27 October 1995, Pages 219-227

https://doi.org/10.1016/0378-1119(95)00396-N Get rights and content

Abstract

We previously established a method, called high-density cDNA filter analysis (HDCFA), for analyzing the expression profiles of a large number of genes in a systematic manner. In the present study, we constructed a cDNA filter of about 8300 cDNAs from a human cerebral cortex cDNA library and quantitatively analyzed their expression in human adult brain, fetal brain, kidney and liver using HDCFA. Using a comparison of the relative amount of expression of each clone in different tissues and following (partial) sequence analysis, about 200 clones were selected as those preferentially expressed in adult or fetal brain, one half of which may be unknown. Their expression was further analyzed in human neuroblastoma cell lines, a human glioma cell line, human cerebral cortex, cerebellum and kidney. Finally, eight clones were selected and sequenced as characteristically expressed genes (cDNAs). A homology search revealed that three clones were human homologues of the rat genes preferentially expressed in brain and five clones were unknown. The full-length cDNA sequence of one of the unknown clones was determined.

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8 Transcript Analysis
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This chapter discusses the basic concepts related to transcript analysis. mRNA abundance measurements provide a useful tool to study gene regulation. Currently, most groups use such measurements to investigate the expression of a small number of specific genes. However, with the advent of genomic sequencing projects and the completion of the yeast genome sequence, genomic transcript analyses are being exploited to generate global pictures of gene regulation. By necessity, genome-scale transcript analyses are focusing mainly on the open reading frames (ORFs) themselves—that is, coding sequences. However, the secondary and tertiary structures formed by these sequences and their 5′-leader and 3′-trailer regions exert strong influences on gene expression at post-transcriptional levels. Hence, these issues are an integral part of transcript analysis. The chapter discusses classical methods for the analysis of specific mRNAs. It describes new technologies that are being developed for gene expression analysis and focuses mainly on mRNA abundance measurements. Methods for the analysis of transcript structure and stability are also discussed in the chapter.

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View full text

High-density cDNA filter analysis of the expression profiles of the genes preferentially expressed in human brain

Abstract

Brain Res.

Neuron

Neuron

Gene

Recongnition of protein coding regions in DNA sequences

Nucleic Acids Res.

Intermediate filaments as mechanical integrators of cellular space

Nature

The identification of a novel synaptosomal-associated protein, SNAP-25, differentially expressed by neuronal subpopulations

J. Cell Biol.