A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F

doi:10.1016/0092-8674(92)90107-N

Cell

Volume 70, Issue 2, 24 July 1992, Pages 337-350

https://doi.org/10.1016/0092-8674(92)90107-N Get rights and content

Abstract

The retinoblastoma protein (pRB) plays an important role in the control of cell proliferation, apparently by binding to and regulating cellular transcription factors such as E2F. Here we describe the characterization of a cDNA clone that encodes a protein with properties of E2F. This clone, RBP3, was identified by the ability of its gene product to interact with pRB. RBP3 bound to pRB both in vitro and in vivo, and this binding was competed by viral proteins known to disrupt pRB-E2F association. RBP3 bound to E2F recognition sequences in a sequence-specific manner. Furthermore, transient expression of RBP3 caused a 10-fold transactivation of the adenovirus E2 promoter, and this transactivation was dependent on the E2F recognition sequences. These properties suggest that RBP3 encodes E2F, or an E2F-like protein.

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Citation Excerpt :
Members of the E2F transcription factor family (E2f1–E2f5) are important regulators of cell proliferation, differentiation, and apoptosis. The most characteristic member of the E2F family is E2f1 [11–13], which controls the initiation of DNA synthesis and the subsequent transition of cells from the G0–G1 to the S phase of the cell cycle [14,15]. In the mouse pancreas, insulin secretion is impaired by an E2f1 deficiency which occurs via the decrease of the K+ channel inward rectifier 6.2 [16].
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Cell

ArticleA cDNA encoding a pRB-binding protein with properties of the transcription factor E2F

Abstract

Cell

Cell

Cell

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Anal. Biochem.

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Cell

Cell

J. Biol. Chem.

J. Mol. Biol.

Cell

Virology

Cell

Cell

J. Mol. Biol.

J. Biol. Chem.

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Cell

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Anal. Biochem.

Gene

Phosphorylation-dependent activation of the adenovirus-inducible E2F factor in a cell-free system

Adenovirus E1a prevents the retinoblastoma gene product from complexing with a cellular transcription factor

Nature

Cyclin A and the retinoblastoma gene product complex with a common transcription factor

Nature

Transcription factor E2F is required for efficient expression of the hamster dihydrofolate reductase gene in vitro and in vivo

Mol. Cell. Biol.

A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels

Eur. J. Biochem.

Suppression of tumorigenicity of human prostate carcinoma cells by replacing a mutated RB gene

Science

Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F

Nature

Empirical prediction of protein conformation

Annu. Rev. Biochem.

Cloning of cDNAs for cellular proteins that bind to the retinoblastoma gene product

Nature

Firefly luciferase gene: structure and expression in mammalian cells

Mol. Cell. Biol.

Identification of germline and somatic mutations affecting the retinoblastoma gene

Science

The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product

Science

Large T antigens of many polyomaviruses are able to form complexes with the retinoblastoma protein

J. Virol.

Adenovirus E1A makes two distinct contacts with the retinoblastoma protein

J. Virol.

Article
A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F