Cell
Article5′ cleavage site in eukaryotic pre-mRNA splicing is determined by the overall 5′ splice region, not by the conserved 5′ GU
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Cited by (162)
Regulation of alternative splicing of PaFT and PaFDL1, the FT and FD homologs in Platanus acerifolia
2022, GeneCitation Excerpt :For the AS of pre-RNA, it is critical to select the appropriate splice sites; the usage of different alternative splice sites leads to different splicing outcomes, thereby affecting the stability of RNA or the function of protein products. The 5′ SS mainly consists of the canonical GT; however, it is determined not by GT dinucleotide but by the whole 5′ splice region, which may be the −3∼+6 region recognized by U1 snRNA using base-pairing at the exon–intron junction (Aebi et al., 1987; Laura et al., 2013). Compared with 5′ SS, the selection and regulation of 3′ SS is more complicated, and 3′ SS is comprised of three consensus motifs (BP, Py, and AG dinucleotide) that are differently conserved, required or spaced among different species (Sohail and Xie, 2015).
Rab GDP-dissociation inhibitor gdiA is an essential gene required for cell wall chitin deposition in Aspergillus niger
2020, Fungal Genetics and BiologyMolecular choreography of pre-mRNA splicing by the spliceosome
2019, Current Opinion in Structural BiologyActivation of a cryptic splice site in a potentially lethal coagulation defect accounts for a functional protein variant
2012, Biochimica et Biophysica Acta - Molecular Basis of DiseaseCitation Excerpt :This has been shown by us in coagulation factor VII (FVII) deficiency [2] and by Pacho and co-workers in LAMA3 deficiency [3]. Mutations at the completely conserved guanine at the intronic + 1 position (Fig. 1A, top) of donor splice sites (5'ss), a relatively frequent cause of severe human genetic diseases [4], are also generally considered null mutations as they are predicted to disrupt the splicing process [5]. However, for most of them, the complete loss-of-function feature has not been demonstrated.
RNA Processing in C. elegans
2011, Methods in Cell BiologyCitation Excerpt :In all organisms, introns containing these splice-site consensus sequences are called GT–AG introns. The splicing reaction happens in multiple steps: First, the U1 snRNP binds to the 5′ splice site of the intron via base-pairing between the U1 snRNP sequence 3′-UC/CAUUCA-5′ and the 5′ splice-site consensus 5′-AG/GURAGU-3′ (Aebi et al., 1987; Séraphin et al., 1988). The two subunits of the U2 snRNP auxillary factor (U2AF), U2AF65 and U2AF35, bind the polypryimidine tract and the AG nucleotides of the 3′ splice site, respectively (Merendino et al., 1999; Wu et al., 1999; Zorio and Blumenthal, 1999a).
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Present address: Max Planck Institut für Molekulare Genetik, Ihnestrasse 63-73, D-1000 Berlin 33, Federal Republic of Germany.