Elsevier

Experimental Cell Research

Volume 101, Issue 2, September 1976, Pages 235-243
Experimental Cell Research

Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells

https://doi.org/10.1016/0014-4827(76)90373-6Get rights and content

Abstract

Cells from four different mouse-human somatic cell hybrids were stained with quinacrine to identify each metaphase chromosome and with ammoniacal silver by the Ag-AS method to locate nucleolus organizer regions. Each of the hybrids contained human acrocentric chromosomes. None of these human acrocentric chromosomes was stained with silver in any hybrid cell. Diploid cells were available from the human parent of one of the hybrids. In these cells both copies of nos. 13 and 15 stained with silver; the same chromosomes in the hybrid cell were not stained. These results support earlier reports that the expression of human ribosomal RNA (rRNA) genes is suppressed in mouse-human hybrid cells. Further, they suggest that silver staining by the Ag-AS method reflects activity of rRNA genes rather than just the presence of these genes.

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    This work was supported by grants from the National Foundation-March of Dimes and the NIH (CA 12504).

    1

    O. J. M. is a Career Scientist of the New York State Health Research Council.

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