The appearance of acetylated α-tubulin during early development and cellular differentiation in Xenopus

doi:10.1016/0012-1606(89)90134-6

Developmental Biology

Volume 136, Issue 1, November 1989, Pages 104-117

https://doi.org/10.1016/0012-1606(89)90134-6 Get rights and content

Abstract

Early development in Xenopus is characterized by dramatic changes in the organization of the microtubule cytoskeleton. We have used whole-mount immunocytochemistry to follow the expression of the acetylated form of α-tubulin during early Xenopus development. In the egg and early embryo, the monoclonal anti-acetylated tubulin antibody 6-11B-1 stained meiotic and mitotic spindles, midbody microtubules, and what appears to be the central region of the sperm aster; the antibody did not stain the sperm aster itself or the cortical microtubule system associated with the rotation of the fertilized egg. Following gastrulation, acetylated tubulin disappeared from all but mitotic midbody microtubules. During the course of neurulation high levels of acetylated tubulin reappeared in the precursors of the ciliated epidermal cells (stage 15), transiently in neural folds (stage $1617$ ), in neuronal processes (stage $1819$ ), and in somas (stage 21). The changing pattern of anti-acetylated tubulin staining during Xenopus development raises intriguing questions as to the physiological significance of tubulin acetylation.

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Full paperThe appearance of acetylated α-tubulin during early development and cellular differentiation in Xenopus

Abstract

Dev. Biol

Dev. Biol

Dev. Biol

Dev. Biol

Cell

Dev. Biol

Dev. Biol

Cell

Dev. Biol

Dev. Biol

Cytoplasmic filaments and morphogenetic movements in the amphibian neural tube

Dev. Biol

Fine structural changes in the differentiating epidermis of Xenopus laevis embryos

J. Anat

Microtubule organizing centers

Annu. Rev. Cell Biol

Microtubules and microfilaments in amphibian neurulation

Amer. Zool

Posttranslational modifications of α-tubulin: Acetylated and detyrosinated forms in axons of rat cerebellum

J. Cell Biol

Fate of secretory proteins trapped in oocytes of Xenopus laevis by disruption of the cytoskeleton or by imbalanced subunit synthesis

J. Cell Biol

Properties of the primary organization field in the embryo of Xenopus laevis. IV. Pattern formation and regulation following early inhibition of mitosis

J. Embryol. Exp. Morphol

A wholemount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus

Development

Studies of nuclear and cytoplasmic behavior during the five mitotic cycles that precede gastrulation in Drosophila embryogenesis

J. Cell Sci

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Postpolymerization detryosination of α-tubulin: A mechanism for subcellular differentiation of microtubules

J. Cell Biol

Synaptic junction development in the spinal cord of an amphibian embryo: An electron microscopic study

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Full paper
The appearance of acetylated α-tubulin during early development and cellular differentiation in Xenopus