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Enhanced production of MMP-1, MMP-3, MMP-13, and RANTES by interaction of chondrocytes with autologous T cells

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Abstract

It has been reported that T cells and chondrocytes interact through cell surface molecules such as MHC, CD4 or CD8 in osteoarthritis (OA) and T cells are activated. The objective of this study is to investigate the responses of chondrocyte–T cell interaction in terms of metalloprotease (MMP) and chemokine production. Articular cartilage and autologous blood were obtained from patients with OA and fracture who under went prosthetic surgery. Synovial fluid (SF) was collected from OA patients. Isolated chondrocytes were co-cultured with autologous T cells. SF cells were analyzed by immunostaining or Alcian blue staining. The production of MMP-1, MMP-3, MMP-13, and regulated on activation, normal T expressed and secreted (RANTES) was enhanced by direct co-culture compared to indirect co-culture using Transwell. Production ratio of RANTES in OA was significantly higher than non-arthritic samples. CD3 positive mononuclear cells and chondrocyte-like cells were found in SF. Chondrocyte–T cell contact was more adhesive in OA samples. These results showed the production of MMPs and RANTES was enhanced by the interaction and that chondrocyte–T cell contact was possible in vivo.

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References

  1. Pelletier JP, Martel-Pelletier J, Abramson SB (2001) Osteoarthritis, an inflammatory disease: potential implication for the selection of new therapeutic targets. Arthritis Rheum 44:1237–1247

    Article  PubMed  CAS  Google Scholar 

  2. Revell PA, Myston V, Lalor P (1988) The synovial membrane in osteoarthritis: a histological study including the characterization of the cellular infiltrate present in inflammatory osteoarthritis. Ann Rheum Dis 47:300–307

    Article  PubMed  CAS  Google Scholar 

  3. Sakkas LI, Scanzello C, Johanson N, Burkholder J, Mitra A, Salgame P, Katsetos CD, Platsoucas CD (1998) T cells and T-cell cytokine transcripts in the synovial membrane in patients with osteoarthritis. Clin Diagn Lab Immunol 5:430–437

    PubMed  CAS  Google Scholar 

  4. Nakamura H, Yoshino S, Kato T, Tsuruha J, Nishioka K (1999) T-cell mediated inflammatory pathway in osteoarthritis. Osteoarthr Cartil 7:401–402

    Article  PubMed  CAS  Google Scholar 

  5. Hansen C, Otto E, Kuhlemann K, Forster G, Kahaly GJ (1996) Glycosaminoglycans in autoimmunity. Clin Exp Rheumatol 14:S59–S67

    PubMed  Google Scholar 

  6. Guerassimov A, Zhang Y, Cartman A, Rosenberg LC, Esdaile J, Fitzcharles MA, Poole AR (1999) Immune responses to cartilage link protein and the G1 domain of proteoglycan aggrecan in patients with osteoarthritis. Arthritis Rheum 42:527–533

    Article  PubMed  CAS  Google Scholar 

  7. Tsuruha J, Masuko-Hongo K, Kato T, Sakata M, Nakamura H, Nishioka K (2001) Implication of cartilage intermediate layer protein in cartilage destruction in subsets of patients with osteoarthritis and rheumatoid arthritis. Arthritis Rheum 44:838–845

    Article  PubMed  CAS  Google Scholar 

  8. Yao Z, Nakamura H, Masuko-Hongo K, Suzuki-Kurokawa M, Nishioka K, Kato T (2004). Characterisation of cartilage intermediate layer protein (CILP)-induced arthropathy in mice. Ann Rheum Dis 63:252–258

    Article  PubMed  CAS  Google Scholar 

  9. Sakata M, Masuko-Hongo K, Tsuruha J, Sekine T, Nakamura H, Takigawa M, Nishioka K, Kato T (2002) YKL-39, a human cartilage-related protein, induces arthritis in mice. Clin Exp Rheumatol 20:343–350

    PubMed  CAS  Google Scholar 

  10. Tsuruha J, Masuko-Hongo K, Kato T, Sakata M, Nakamura H, Sekine T, Takigawa M, Nishioka K (2002) Autoimmunity against YKL-39, a human cartilage derived protein, in patients with osteoarthritis. J Rheumatol 29:1459–1466

    PubMed  CAS  Google Scholar 

  11. Lance EM, Kimura LH, Manibog CN (1993) The expression of major histocompatibility antigens on human articular chondrocytes. Clin Orthop 291:266–282

    PubMed  Google Scholar 

  12. Alsalameh S, Jahn B, Krause A, Kalden JR, Burmester GR (1991) Related articles, links antigenicity and accessory cell function of human articular chondrocytes. J Rheumatol 18:414–421

    PubMed  CAS  Google Scholar 

  13. Sakata M, Masuko-Hongo K, Nakamura H, Onuma H, Tsuruha JI, Aoki H, Nishioka K, Kato T (2003) Osteoarthritic articular chondrocytes stimulate autologous T cell responses in vitro. Clin Exp Rheumatol 21:704–710

    PubMed  CAS  Google Scholar 

  14. Billinghurst RC, Dahlberg L, Ionescu M et al (1997) Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage. J Clin Invest 99:1534–1545

    Article  PubMed  CAS  Google Scholar 

  15. Okada Y, Shinmei M, Tanaka O, Naka K, Kimura A, Nakanishi I, Bayliss MT, Iwata K, Nagase H (1992) Localization of matrix metalloproteinase 3 (stromelysin) in osteoarthritic cartilage and synovium. Lab Invest 66:680–690

    PubMed  CAS  Google Scholar 

  16. Nakamura H, Shibakawa A, Tanaka M, Kato T, Nishioka K (2004) Effects of glucosamine hydrochloride on the production of prostaglandin E2, nitric oxide and metalloproteases by chondrocytes and synoviocytes in osteoarthritis. Clin Exp Rheumatol 22:293–299

    PubMed  CAS  Google Scholar 

  17. Yuan GH, Masuko-Hongo K, Sakata M, Tsuruha J, Onuma H, Nakamura H, Aoki H, Kato T, Nishioka K (2001) The role of C–C chemokines and their receptors in osteoarthritis. Arthritis Rheum 44:1056–1070

    Article  PubMed  CAS  Google Scholar 

  18. Altman R, Asch E, Bloch D, Bole G, Borenstein D, Brandt K, Christy W, Cooke TD, Greenwald R, Hochberg M, Howell D, Kaplan D, Koopman W, Longley III S, Mankin H, McShane DJ, Medsger Jr T, Meenan R, Mikkelsen W, Moskowitz R, Murphy W, Rothschild B, Segal M, Sokoloff L, Wolfe F (1986) Development of criteria for the classification and reporting of osteoarthritis. Classification of osteoarthritis of the knee. Diagnostic and therapeutic criteria committee of the American rheumatism association. Arthritis Rheum 29:1039–1049

    Article  PubMed  CAS  Google Scholar 

  19. Jobanputra P, Corrigall V, Kingsley G, Panayi G (1992) Cellular responses to human chondrocytes: absence of allogeneic responses in the presence of HLA-DR and ICAM-1. Clin Exp Immunol 90:336–344

    Article  PubMed  CAS  Google Scholar 

  20. Kienzle G, Kempis J (1998) Vascular cell adhesion molecule 1 (CD106) on primary human articular chondrocytes. Arthritis Rheum 41:1296–1305

    Article  PubMed  CAS  Google Scholar 

  21. Horner A, Davies ME, Franz B (1995) Chondrocyte-peripheral blood mononuclear cell interactions: the role of ICAM-1. Immunol 86:584–590

    CAS  Google Scholar 

  22. Ashman RF (1994) Turek’s orthopaedics, principles and their application, 5th edn. In: Ryan JD (ed) Lippincott Co., Philadephia, pp 152–155

  23. Hashimoto S, Ochs RL, Komiya S, Lotz M (1998) Linkage of chondrocyte apoptosis and cartilage degradation in human osteoarthritis. Arthritis Rheum 41:1632–1638

    Article  PubMed  CAS  Google Scholar 

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Acknowledgement

This study is partly supported by the Japanese Ministry of Education, Culture, Sports, Science and Technology.

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Authors and Affiliations

  1. Institute of Medical Science, St. Marianna University, 2-16-1, Miyamae-ku, Sugao, 216-8512, Kawasaki, Japan

    Hiroshi Nakamura, Michiaki Tanaka, Kayo Masuko-Hongo, Kazuo Yudoh, Tomohiro Kato & Kusuki Nishioka

  2. Department of Orthopedics, St. Marianna University, 2-16-1, Miyamae-ku, Sugao, 216-8512, Kawasaki, Japan

    Moroe Beppu

Authors
  1. Hiroshi Nakamura
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  2. Michiaki Tanaka
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  3. Kayo Masuko-Hongo
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  4. Kazuo Yudoh
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  5. Tomohiro Kato
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  6. Moroe Beppu
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  7. Kusuki Nishioka
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Correspondence to Hiroshi Nakamura.

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Nakamura, H., Tanaka, M., Masuko-Hongo, K. et al. Enhanced production of MMP-1, MMP-3, MMP-13, and RANTES by interaction of chondrocytes with autologous T cells. Rheumatol Int 26, 984–990 (2006). https://doi.org/10.1007/s00296-006-0116-5

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  • DOI: https://doi.org/10.1007/s00296-006-0116-5

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