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Structure, chromosomal localization, and expression of 12 genes of the MAGE family

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Abstract

We reported previously that human geneMAGE-1 directs the expression of a tumor antigen recognized on a melanoma by autologous cytolytic T lymphocytes. Probing cosmid libraries with aMAGE-1 sequence, we identified 11 closely related genes. The analysis of hamster-human somatic cell hybrids indicated that the 12MAGE genes are located in the q terminal region of chromosome X. LikeMAGE-1, the 11 additionalMAGE genes have their entire coding sequence located in the last exon, which shows 64%-85% identity with that ofMAGE-1. The coding sequences of theMAGE genes predict the same main structural features for allMAGE proteins. In contrast, the promoters and first exons of the12 MAGE genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The expression of eachMAGE gene was evaluated by reverse transcription and polymerase chain reaction amplification. Six genes of theMAGE family includingMAGE-1 were found to be expressed at a high level in a number of tumors of various histological types. None was expressed in a large panel of healthy tissues, with the exception of testis and placenta.

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References

  • Anichini, A., Fossati, G., and Parmiani, G. Clonal analysis of the cytolytic T -cell response to human tumors.Immunol Today 8: 385–389, 1987

    Google Scholar 

  • Brasseur, F., Marchand, M., Vanwijck, R., Hérin, M., Lethé, B., Chomez, P., and Boon, T. Human gene MAGE-1, which codes for a tumor rejection antigen, is expressed by some breast tumors.Int J Cancer 52: 839–841, 1992

    Google Scholar 

  • De Smet, C., Lurquin, C., van der Bruggen, P., De Plaen, E., Brasseur, F., and Boon, T. Sequence and expression pattern of the human MAGE2 gene.Immunogenetics 39: 121–129, 1994

    Google Scholar 

  • Falk, K., Rötzschke, O., Stevanovic, S., Jung, G., and Rammensee, H. -G. Allele-specific motifs revealed by sequencing of self-peptides eluted from MHC molecules.Nature 351: 290–296, 1991

    Google Scholar 

  • Gaboriaud, C., Bissery, V., Benchetrit, T., and Mornon, J. P. Hydrophobic cluster analysis: an efficient new way to compare and analyse amino acid sequences.FEBS Lett 224: 149–155, 1987

    Google Scholar 

  • Gaugler, B., van den Eynde, B., van der Bruggen, P., Romero, P., Gaforio, J. J., De Plaen, E., Lethé, B., Brasseur, F., and Boon, T. Human gene MAGE-3 codes for an antigen recognized on a melanoma by autologous cytolytic T lymphocytes.J Exp Med 179: 921–930, 1994

    Google Scholar 

  • Hérin, M., Lemoine, C., Weynants, P., Vessière, E., Van Pel, A., Knuth, A., Devos, R., and Boon, T. Production of stable cytolytic T-cell clones directed against autologous human melanoma.Int J Cancer 39: 390–396, 1987

    Google Scholar 

  • Hill, A. V. S., Elvin, J., Willis, A. C., Aidoo, M., Allsopp, C. E. M., Gotch, F. M., Gao, X. M., Takiguchi, M., Greenwood, B. M., Townsend, A. R. M., and McMichael, A. J. Molecular analysis of the association of HLA-B53 and resistance to severe malaria.Nature 360: 434–439, 1992

    Google Scholar 

  • Houbiers, J. G. A., Nijman, H. W., van der Burg, S. H., Drijfhout, J. W., Kenemans, P., van de Velde, C. J. H., Brand, A., Momburg, F., Kast, M. W., and Melief, C. J. M. In vitro induction of human cytotoxic T lymphocyte responses against peptides of mutant and wild-type p53.Eur J Immunol 23: 2072–2077, 1993

    Google Scholar 

  • James, C. D., Carlbom, E., Dumanski, J. P., Hansen, M., Nordenskjold, M., Collins, V. P., and Cavenee, W. K. Clonal genomic alterations in glioma malignancy stages.Cancer Res 48: 5546–5551, 1988

    Google Scholar 

  • Jardetzky, T. S., Lane, W. S., Robinson, R. A., Madden, D. R., and Wiley, D. C. Identification of self peptides bound to purified HLAB27.Nature 353: 326–329, 1991

    Google Scholar 

  • Knuth, A., Danowski, B., Oettgen, H. F., and Old, L. T-cell mediated cytotoxicity against autologous malignant melanoma: analysis with interleukin-2-dependent T-cell cultures.Proc Natl Acad Sci USA 81: 3511–3515, 1984

    Google Scholar 

  • Maruyama, K., Usami, M., Aizawa, T., and Yoshikawa, K. A novel brain-specific mRNA encoding nuclear protein (necdin) expressed in neurally differentiated embryonal carcinoma cells.Biochem Biophys Res Commun 178: 291–296, 1991

    Google Scholar 

  • McMichael, A. J., Gotch, F. M., and Rothbard, J. HLA B37 determines an influenza A virus nucleoprotein epitope recognized by cytotoxic T lymphocytes.J Exp Med 164: 1397–1406, 1986

    Google Scholar 

  • Mukherji, B. and MacAlister, T. J. Clonal analysis of cytotoxic T cell response against human melanoma.J Exp Med 158: 240–245, 1983

    Google Scholar 

  • Taylor, P. M., Davey, J., Howland, K., Rothbard, J. B., and Askonas, B. A. Class I MHC molecules rather than other mouse genes dictate influenza epitope recognition by cytotoxic T cells.Immunogenetics 26: 267–272, 1987

    Google Scholar 

  • Traversari, C., van der Bruggen, P., Luescher, I. F., Lurquin, C., Chomez, P., Van Pel, A, De Plaen, E., Amar-Costesec, A., and Boon, T. A nonapeptide encoded by human gene MAGE-1 is recognized on HLA-A1 by cytolytic T lymphocytes directed against tumor antigen MZ2-E.J Exp Med 176: 1453–1457, 1992a

    Google Scholar 

  • Traversari, C., van der Bruggen, P., Van den Eynde, B., Hainaut, P., Lemoine, C., Ohta, N., Old, L., and Boon, T. Transfection and expression of a gene coding for a human melanoma antigen recognized by autologous cytolytic T lymphocytes.Immunogenetics 35: 145–152, 1992b

    Google Scholar 

  • Van den Eynde, B., Hainaut, P., Hérin, M., Knuth, A., Lemoine, C., Weynants, P., van der Bruggen, P., Fauchet, R., and Boon, T. Presence on a human melanoma of multiple antigens recognized by autologous CTL.Int J Cancer 44: 634–640, 1989

    Google Scholar 

  • Van der Bruggen, P, Szikora, J.-P, Boël, P., Wildmann, C., Somville, M., Sensi, M., and Boon, T. Autologous cytolytic T lymphocytes recognize a MAGE-1 nonapeptide on melanomas expressing HLA-Cw*1601.Eur J Immunol, in press

  • Van der Bruggen, P., Traversari, C., Chomez, P., Lurquin, C., De Plaen, E., Van den Eynde, B., Knuth, A., and Boon, T. A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma.Science 254: 1643–1647, 1991

    Google Scholar 

  • Weynants, P., Lethé, B., Brasseur, F., Marchand, M., and Boon, T. Expression of MAGE genes by non-small-cell lung carcinomas.Int J Cancer 56: 826–829, 1994

    Google Scholar 

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De Plaen, E., Traversari, C., Gaforio, J.J. et al. Structure, chromosomal localization, and expression of 12 genes of the MAGE family. Immunogenetics 40, 360–369 (1994). https://doi.org/10.1007/BF01246677

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