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The X-linked methylated DNA binding protein, Mecp2, is subject to X inactivation in the mouse

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Abstract

DNA methylation at the promoter region of X-linked genes is associated with the maintenance of X inactivation in mammals. One of the methylated DNA binding proteins, MECP2, that binds to methylated bases in DNA is encoded by a gene (Mecp2) located on the mouse X Chromosome (Chr). To determine whether this gene was expressed from the inactive X Chr, and X-autosome translocation (T(X;16)16H) system in which expression from the Mecp2 allele on the inactive X Chr could be assayed was used. Results from these experiments indicate that Mecp2 is subject to X inactivation in mouse.

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References

  • Adler, D.A., Bressler, S.L., Chapman, V.M., Page, D.C., Disteche, C.M. (1991). Inactivation of the Zfx gene on the mouse X chromosome. Proc. Natl. Acad. Sci. USA 88, 4592–4595.

    Google Scholar 

  • Boyes, J., Bird, A. (1991). DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein. Cell 64, 1123–1134.

    Google Scholar 

  • Brown, S.D.M., Avner, P., Boyd, Y., Chapman, V., Rastan, S., Sefton, L., Thomas, J.D., Herman, G.E. (1993). Mouse X chromosome. Mammalian Genome 4(Suppl.), S269-S281.

    Google Scholar 

  • Chomzynski, P., Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156–159.

    Google Scholar 

  • Lewis, J.D., Meehan, R.R., Henzel, W.J., Maurer-Fogy, I., Jeppesen, P., Klein, F., Bird, A. (1992). Purification, sequence, and cellular localization of a novel chromosomal protein that binds to methylated DNA. Cell 69, 905–914.

    Google Scholar 

  • Lyon, M.F., Searle, A.G., Ford, C.E., Ohno, S. (1964) A mouse translocation suppressing sex-linked variegation. Cytogenetics 3, 306–323.

    Google Scholar 

  • Meehan, R.R., Lewis, J.D., McKay, S., Kleiner, E.L., Bird, A.P. (1989). Identification of a mammalian protein that binds specifically to DNA containing methylated CpGs. Cell 58, 499–507.

    Google Scholar 

  • Meehan, R.R., Lewis, J.D., Bird, A.P. (1992). Characterization of MeCP2, a vertebrate DNA binding protein with affinity for methylated DNA. Nucleic Acids Res. 20, 5085–5092.

    Google Scholar 

  • Quaderi, N.A., Meehan, R., Tate, P.H., Cross, S.H., Bird, A.P., Chatterjee, A., Herman, G.E., Brown, S.D.M. (1994). Genetic and physical mapping of a gene encoding a methyl CpG binding protein, Mecp2, to the mouse X chromosome. Genomics 22, 648–651.

    Google Scholar 

  • Searle, A.G. (1962). Is sex-linked Tabby really recessive in the mouse? (abstract). Heredity 17, 297.

    Google Scholar 

  • Singer-Sam, J., Riggs, A.D. (1993). X-chromosome inactivation and DNA methylation. Exper. Suppl. (Basel). 64, 358–384.

    Google Scholar 

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Adler, D.A., Quaderi, N.A., Brown, S.D.M. et al. The X-linked methylated DNA binding protein, Mecp2, is subject to X inactivation in the mouse. Mammalian Genome 6, 491–492 (1995). https://doi.org/10.1007/BF00356163

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  • DOI: https://doi.org/10.1007/BF00356163

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