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Patterns of exchange induced by mitomycin C in C-bands of human chromosomes. I. Relationship to C-band size in chromosomes 1, 9, and 16

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Summary

Frequencies of exchange were determined in C-bands of chromosomes 1, 9 and 16 in six normal males, and related to relative C-band area. Comparing these different chromosomes, more exchanges occurred on average in 9 than in 1 although their mean C-band sizes were similar. Chromosome 16 exchanges were fewer, both overall and relative to C-band area. Comparing the same chromosome between individuals, there was a positive correlation between relative frequency and band size in both 1-1 and 9-9 exchanges. No clear trend was observed for other exchange events.

If homology is required for interchange, if cannot be dependent solely on overall C-band size. Perhaps certain DNA sequences, sensitive to mitomycin C damage, are located in part of each C-band, with less per unit area in chromosome 1 than in 9 and still less in chromosome 16.

X- and U-type exchanges between chromosome 9s occurred in near equal frequencies in all individuals. If synapsis of specific, affected sequences is a pre-requisite for interchange, this observation suggests that the affected sequence in chromosome 9 is arranged in both orientations relative to the centromere.

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References

  • Angell RR, Jacobs PA (1978) Lateral asymmetry in human constitutive heterochromatin: frequency and inheritance. Am J Hum Genet 30: 144–152

    Google Scholar 

  • Balíček P, Zižka J, Skalská H (1978) Variability and familial transmission of constitutive heterochromatin of human chromosomes evaluated by the method of linear measurement. Hum Genet 42: 257–265

    Google Scholar 

  • Bourgeois CA (1974) Distribution of mitomycin C-induced damage in human chromosomes with special reference to regions of repetitive DNA. Chromosoma 48:203–211

    Google Scholar 

  • Brøgger A, Johansen J (1972) A model for the production of chromosome damage by mitomycin C. Chromosoma 38:95–104

    Google Scholar 

  • Cohen MM, Shaw MW (1964) Effects of mitomycin C on human chromosomes. J Cell Biol 23:386–395

    Google Scholar 

  • Comings DE (1975) Implications of somatic recombination and sister chromatid exchange in Bloom's syndrome and cells treated with mitomycin C. Humangenetik 28:191–196

    Google Scholar 

  • Cooke HJ, Hindley J (1979) Cloning of human satellite III DNA: different components are on different chromosomes. Nucleic Acids Res 6:3177–3197

    Google Scholar 

  • Donlon TA, Magenis RE (1981) Structural organization of the heterochromatic region of human chromosome 9. Chromosoma 84: 353–363

    Google Scholar 

  • Gosden JR, Mitchell AR, Buckland RA, Clayton RP, Evans HJ (1975) The location of four human satellite DNAs on human chromosomes. Exp Cell Res 92:148–158

    Google Scholar 

  • Hayman D (1980) Factors affecting the frequency and pattern of mitomycin C-induced chromatid exchanges. Chromosoma 78: 341–352

    Google Scholar 

  • Hoehn H, Martin GM (1973) Nonrandom arrangement of human chromatin: topography of disomic markers X, Y, and 1h+. Cytogenet Cell Genet 12:443–452

    Google Scholar 

  • Joseph JL, Brasch JM, Smyth DR (1983) Patterns of exchange induced by mitomycin C in C-bands of human chromosomes. II. High frequency of Y-Y exchange in XYY cells. Hum Genet (in press)

  • Mitchell AR, Beauchamp RS, Bostock CJ (1979) A study of sequence homologies in four satellite DNAs of man. J Mol Biol 135:127–149

    Google Scholar 

  • Morad M, Jonasson J, Lindsten J (1973) Distribution of mitomycin C induced breaks on human chromosomes. Hereditas 74:273–282

    Google Scholar 

  • Schaap T, Sagi M, Cohen MM (1980) Chromosome-specific patterns of mitomycin C-induced rearrangements in human lymphocytes. Cytogenet Cell Genet 28:240–250

    Google Scholar 

  • Schmid M, Vogel W, Krone W (1975) Attraction between centric heterochromatin of human chromosomes. Cytogenet Cell Genet 15:66–80

    Google Scholar 

  • Shaw MW, Cohen MM (1965) Chromosome exchanges in human leukocytes induced by mitomycin C. Genetics 51:181–190

    Google Scholar 

  • Spaeter M (1975) Nichtzufällige Verteilung homologer Chromosomen (Nr. 9 und YY) in Interphasekernen menschlicher Fibroblasten. Humangenetik 27:111–118

    Google Scholar 

  • Stahl A, Hartung M, Vagner-Capodano AM, Fouet C (1976) Chromosomal constitution of nucleolus-associated chromatin in man. Hum Genet 35:27–34

    Google Scholar 

  • Sumner AT (1972) A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res 75:304–306

    Google Scholar 

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Authors and Affiliations

  1. Department of Genetics, Monash University, 3168, Clayton, Victoria, Australia

    J. L. Joseph, J. M. Brasch & D. R. Smyth

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  1. J. L. Joseph
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  2. J. M. Brasch
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  3. D. R. Smyth
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Joseph, J.L., Brasch, J.M. & Smyth, D.R. Patterns of exchange induced by mitomycin C in C-bands of human chromosomes. I. Relationship to C-band size in chromosomes 1, 9, and 16. Hum Genet 62, 342–345 (1982). https://doi.org/10.1007/BF00304552

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  • DOI: https://doi.org/10.1007/BF00304552

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