Elsevier

Genomics

Volume 54, Issue 2, 1 December 1998, Pages 191-199
Genomics

Regular Article
The Human Gene Encoding the Lectin-Type Oxidized LDL Receptor (OLR1) Is a Novel Member of the Natural Killer Gene Complex with a Unique Expression Profile

https://doi.org/10.1006/geno.1998.5561Get rights and content

Abstract

LOX-1 is an endothelial receptor for oxidized low-density lipoprotein that plays essential roles in atherogenesis. LOX-1 has the highest homology with C-type lectin receptors expressed on natural killer cells. In the present study, we cloned and characterized the human LOX-1 gene (HGMW-approved symbol OLR1). The gene structure of LOX-1 resembles that of the natural killer cell receptors. Fluorescencein situhybridization and analyses of a yeast artificial chromosome contig revealed that the human LOX-1 gene is located in the natural killer gene complex on chromosome 12p12–p13, where the genes of the natural killer cell receptors cluster. In contrast, the expression pattern of LOX-1 is different from that of the natural killer cell receptors; LOX-1 is expressed in vascular-rich organs, but not in lymphocytes. A 1753-bp fragment of the 5′ flanking region of the LOX-1 gene had a functional promoter activity. This region contains binding sites for several transcription factors, including the STAT family and NF-IL6, and the expression of LOX-1 was upregulated by several cytokines. These results demonstrate that the human LOX-1 gene is a new member of the natural killer gene complex with a unique expression profile.

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    Sequence data from this article have been deposited with the GenBank Data Library under Accession Nos. AF079164, AF079165, AF079166, and AF079167.

    1

    To whom correspondence should be addressed at the Department of Pharmacology, Osaka City University Medical School, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan. Telephone: 81-6-645-3731. Fax: 81-6-646-1980. E-mail:[email protected].

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