Elsevier

Genomics

Volume 49, Issue 2, 15 April 1998, Pages 327-330
Genomics

Short Communication
Cloning, Expression, and Chromosomal Localization of Human Long-Chain Fatty Acid-CoA Ligase 4 (FACL4)

https://doi.org/10.1006/geno.1998.5268Get rights and content

Abstract

Long-chain fatty acid-CoA ligase (also called fatty acid acyl-CoA synthetase) plays an essential role in lipid biosynthesis and fatty acid degradation. We report herein the cDNA cloning of the human long-chain fatty acid-CoA ligase 4 from a brain library. The cDNA encodes a functional long-chain fatty acid-CoA ligase that shows preference for arachidonic acid as substrate. We also studied the tissue distribution of gene expression by Northern hybridization. Human placenta, brain, testis, ovary, spleen, and adrenal cortex have the highest levels of expression of the long-chain fatty acid-CoA ligase 4, whereas the GI system has the lowest. Finally, this gene was localized to chromosome Xq23 in human by FISH analysis.

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Sequence data from this article have been deposited with the GenBank Data Library under Accession No. AF030555.

1

To whom correspondence should be addressed at the Huntsman Cancer Institute, University of Utah, 15 North 2030 East, Suite 4220, Salt Lake City, UT 84112-5330. Telephone: (801) 585-3401. Fax: (801) 585-6345. E-mail:[email protected].

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