Elsevier

Genomics

Volume 47, Issue 1, 1 January 1998, Pages 26-43
Genomics

Regular Article
High-Resolution YAC–Cosmid–STS Map of Human Chromosome 13

https://doi.org/10.1006/geno.1997.5087Get rights and content
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Abstract

We have assembled a high-resolution physical map of human chromosome 13 DNA (∼114 Mb) from hybridization, PCR, and FISH mapping data using a specifically designed set of computer programs. Although the mapping of 13p is limited, 13q (∼98 Mb) is covered by an almost continuous contig of 736 YACs aligned to 597 contigs of cosmids. Of a total of 10,789 cosmids initially selected from a chromosome 13-specific cosmid library (16,896 colonies) using inter-AluPCR probes from the YACs and probes for markers mapped to chromosome 13, 5111 were assembled in contigs that were established from cross-hybridization relationships between the cosmids. The 13q YAC–cosmid map was annotated with 655 sequence tagged sites (STSs) with an average spacing of 1 STS per 150 kb. This set of STSs, each identified by a D number and cytogenetic location, includes database markers (198), expressed sequence tags (93), and STSs generated by sequencing of the ends of cosmid inserts (364). Additional annotation has been provided by positioning 197 cosmids mapped by FISH on 13q. The final (comprehensive) map, a list of STS primers, and raw data used in map assembly are available at our Web site (genome1.ccc.columbia.edu/∼genome/) and can serve as a resource to facilitate accurate localization of additional markers, provide substrates for sequencing, and assist in the discovery of chromosome 13 genes associated with hereditary diseases.

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