Elsevier

Genomics

Volume 34, Issue 1, 15 May 1996, Pages 1-8
Genomics

Regular Article
Chromosomal Assignment of Human DNA Fingerprint Sequences by Simultaneous Hybridization to Arbitrarily Primed PCR Products from Human/Rodent Monochromosome Cell Hybrids

https://doi.org/10.1006/geno.1996.0235Get rights and content

Abstract

We have developed a technique for the simultaneous chromosomal assignment of multiple human DNA sequences from DNA fingerprints obtained by the arbitrarily primed polymerase chain reaction (AP-PCR). Radioactively labeled human AP-PCR products are hybridized to DNA fingerprints generated with the same arbitrary primer from human/rodent monochromosome cell hybrids after electroblotting to a nylon membrane. Human-specific hybridization bands in the human/rodent fingerprints unambiguously determine their chromosome of origin. We named this method simultaneous hybridization of arbitrarily primed PCR DNA fingerprinting products (SHARP). Using this approach, we determined the chromosomal origins of most major bands of human AP-PCR fingerprints obtained with two arbitrary primers. Altogether, the chromosomal localization of near 50 DNA fragments, comprehensive of all human chromosomes except chromosomes 21 and Y, was achieved in this simple manner. Chromosome assignment of fingerprint bands is essential for molecular karyotyping of cancer by AP-PCR DNA fingerprinting. The SHARP method provides a convenient and powerful tool for this purpose.

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1

Present address: Universitat Autonoma de Barcelona, Departament de Genetica I de Microbiologia, 08193 Bella Terra, Barcelona, Spain.

2

Present address: Institut de Recerca Oncologica (IRO), Hospital Durans i Reynals, Autovia de Castelldefels, km 2,7, L'Hospitalet de Llobregat, 08907 Barcelona, Spain.

3

To whom correspondence should be addressed. Telephone: (619) 646-3112. Fax: (619) 646-3190.

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