Regular ArticleAutomation of Genetic Linkage Analysis Using Fluorescent Microsatellite Markers
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Microsatellite and mitochondrial DNA markers unveil the genetic structure of Nellore Palla sheep of India
2022, Small Ruminant ResearchCitation Excerpt :Capillary electrophoresis is advantageous over agarose gel electrophoresis due to its ability to measure the size of PCR products with very high resolution (WHO (World Health Organization) and the Medicines for Malaria Venture, 2007; Liljander et al., 2009). Fluorescence-based microsatellite loci detection and allele scoring on an automated DNA fragment analyser is one of the most accurate methods for genotyping as overlapping alleles are co-separated by labelling with different fluorophores and multi-pooling (Mansfield et al., 1994; Heller, 2001). The value observed over the range of loci in the present study could be regarded as a reasonable indicator of genetic variation within the populations (MacHugh et al., 1997).
A multiplex microsatellite PCR method for evaluating genetic diversity in grass carp (Ctenopharyngodon idellus)
2018, Aquaculture and FisheriesCitation Excerpt :The use of capillary electrophoresis with fluorescently labelled primers provides high detection sensitivity of amplified DNA fragments (Huang, Huang, Hu, & Chang, 2006; Ramachandran et al., 2003; Shi et al., 2003). Multiplex SSR-PCR as an effective, economical and accurate approach for genotyping and germplasm evaluation, it enhances the visualization of polymorphic DNA fragments and was the excellent option of constructed the method for evaluating genetic information of grass carp (Archak, Lakshminarayanareddy, & Nagaraju, 2007; Mansfield et al., 1994; Ziegle et al., 1992). In this study, we constructed a multiplex SSR-PCR detection method possessed high polymorphism and heterozygosity.
Guidelines for the selection of functional assays to evaluate the hallmarks of cancer
2016, Biochimica et Biophysica Acta - Reviews on CancerCitation Excerpt :SNP arrays are capable of detecting copy-neutral loss of heterozygosity (so-called uniparental disomy or gene conversion) and distinguish alleles at specific polymorphic sites [189]. To detect microsatellite instability (MSI), the gold standard approach amplifies known microsatellite regions by PCR and quantifies the lengths of PCR products by gel or capillary electrophoresis using autoradiography, silver staining [190] or fluorescent methods [191,192]. The microsatellite length of the sample is compared to normal DNA to determine MSI status.
Multiplex SSR-PCR approaches for semi-automated genotyping and characterization of loci linked to blast disease resistance genes in rice
2015, Comptes Rendus - BiologiesCitation Excerpt :The use of capillary electrophoresis with fluorescently labelled primers provides high detection sensitivity of amplified DNA fragments [33–35]. This procedure is an effective, economical and accurate approach for genotyping, germplasm evaluation and enhances the visualisation of polymorphic DNA fragments, representing multiple loci from across the genome [36–38]. Nowadays, this method has been developed and used for several plant species, such as Oryza sativa [39–41].
Undetected genotyping errors cause apparent overtransmission of common alleles in the transmission/disequilibrium test
2003, American Journal of Human GeneticsMicrosatellite markers and genetic mapping in Plasmodium falciparum
2000, Parasitology Today