Localization of the Human β-Catenin Gene (CTNNB1) to 3p21: A Region Implicated in Tumor Development

doi:10.1006/geno.1994.1493

Genomics

Volume 23, Issue 1, 1 September 1994, Pages 272-274

https://doi.org/10.1006/geno.1994.1493 Get rights and content

Abstract

The human β-catenin locus (CTNNB1) was mapped by in situ fluorescence analysis to band p21 on the short arm of chromosome 3, a region frequently affected by somatic alterations in a variety of tumors. PCR primers for the genomic amplification of β-catenin sequences were selected on the basis of homology to exon 4 of the Drosophila armadillo gene. Analysis of a panel of somatic cell hybrids confirmed the localization of β-catenin on human chromosome 3. Furthermore, exclusion mapping of three hybrids carrying defined fragments of the short arm of human chromosome 3 allowed us to determine the position of the CTNNB1 locus close to the marker D3S2 in 3p21.

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Recent studies show the increased evidence Wnt/β-Catenin signaling activation associated with high TNBC progression among other mechanisms [1–3]. β-Catenin is a multifunctional protein located to the intracellular side of the cytoplasmic membrane coded by the CTNNB1 gene, which maps to chromosome 3p22.1 [4]. The general structure of β-Catenin comprises of three domains, (i) the N - terminal (residues:1–137) domain initiate signaling cascade, (ii) a central domain (residues: 138–664) combined with 12 armadillo repeat domain (R1-R12) also known as ARM assembled with helices and superhelices via fold back mechanism for cell adhesion (iii) the C - terminal (residues: 665–781) domains are believed to regulating proteasomal degradation [5–7].
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Citation Excerpt :
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Short CommunicationLocalization of the Human β-Catenin Gene (CTNNB1) to 3p21: A Region Implicated in Tumor Development

Abstract

Short Communication
Localization of the Human β-Catenin Gene (CTNNB1) to 3p21: A Region Implicated in Tumor Development