Tool | Prediction | Score/value |
Function-based predictions | ||
Sift | Deleterious | 0.023* |
PolyPhen2 HDIV | Probably damaging | 0.971† |
Mutation Taster | Disease-causing | 0.954‡ |
Structure-based predictions | ||
DynaMut | Destabilising | ΔΔG=−0.947 kcal/mol |
Normal mode analysis (ENCoM) | Destabilising | ΔΔG=−0.315 kcal/mol |
Vibrational entropy energy (ENCoM) (mutant vs wild-type) | Increase of molecule flexibility | ΔΔSVib=0.394 kcal mol-1 K-1 |
mCSM | Destabilising | ΔΔG=−1.500 kcal mol-1 |
SDM | Destabilising | ΔΔG=−0.910 kcal mol-1 |
DUET | Destabilising | ΔΔG=−1.315 kcal mol-1 |
*Deleterious:<=0.05; Tolerated:>0.05.
†Probably damaging: ≥0.957; possibly damaging:0.453≤score≤0.956; benign: ≤0.452.
‡Values close to 1 indicate high 'security' of prediction.