Table 2

Prediction of V1251 effects on CCNB3 function and structure

ToolPredictionScore/value
Function-based predictions
 SiftDeleterious0.023*
 PolyPhen2 HDIVProbably damaging0.971†
 Mutation TasterDisease-causing0.954‡
Structure-based predictions
 DynaMutDestabilisingΔΔG=−0.947 kcal/mol
 Normal mode analysis (ENCoM)DestabilisingΔΔG=−0.315 kcal/mol
 Vibrational entropy energy (ENCoM) (mutant vs wild-type)Increase of molecule flexibilityΔΔSVib=0.394 kcal mol-1 K-1
 mCSMDestabilisingΔΔG=−1.500 kcal mol-1
 SDMDestabilisingΔΔG=−0.910 kcal mol-1
 DUETDestabilisingΔΔG=−1.315 kcal mol-1
  • *Deleterious:<=0.05; Tolerated:>0.05.

  • †Probably damaging: ≥0.957; possibly damaging:0.453≤score≤0.956; benign: ≤0.452.

  • ‡Values close to 1 indicate high 'security' of prediction.