Mutation-positive samples
| Gene | 2011–2016 (n (%)) | Cohort 1: 2011–Oct 2014 (1–5 genes) (n (%)) | Cohort 2: Oct 2014–2016 (NGS panel) (n (%)) |
| PTPN11 | 27/424 (6.4) | 13/231 (5.6) | 14/193 (7.3) |
| RAF1 | 5/424 (1.2) | 1/231 (0.4) | 4/193 (2) |
| RIT1 | 3/424 (0.7) | N/A | 3/193 (1.6) |
| SOS1 | 1/424 (0.2) | N/A | 1/193 (0.5) |
| HRAS | 1/424 (0.2) | N/A | 1/193 (0.5) |
| MAP2K1 | 1/424 (0.2) | 1/231 (0.4) | 0/193 (0) |
| BRAF | 1/424 (0.2) | 0/231 (0) | 1/193 (0.5) |
| SHOC2 | 1/424 (0.2) | N/A | 1/193 (0.5) |
| KRAS | 0/424 (0) | 0/231 (0) | 0/193 (0) |
| All other genes in NGS panel | 0/424 (0) | N/A | 0/193 (0) |
| Total mutation positive | 40/424 (9.4%) | 15/231 (6.5%) | 25/193 (13%) |
N/A, not applicable; NGS, next generation sequencing.