Table 1

Oocyte and embryo characteristics of IVF and ICSI attempts of eight probands

IndividualIVF and ICSI attemptsTotal oocytesMII oocytesFertilised oocytesNormal cleavage embryosViable embryos on day 3Outcomes
Family 1First IVF191514124The four viable embryos (two grade II 7 cells and two grade II 6 cell) were transferred into the uterus later but failed to establish pregnancy; five embryos at 4–5 cell stage were further cultured but failed to form a blastocyst on day 5; the remaining three embryos were arrested at the 2–3 cell stage on day 3.
Second ICSI15111090One embryo was arrested at the two-cell stage and the other eight embryos (including a grade II eight-cell embryo and a grade II seven-cell embryo) were further cultured but failed to form a blastocyst on day 5.
Family 2First IVF129760All embryos were arrested at the 2–7 cell stage on day 3.
Second IVF14141290All embryos were arrested at the 2–7 cell stage on day 3.
Family 3First ICSI22110The embryo was arrested at four-cell stage on day 3.
Second ICSI44210The embryo was failed to form a blastocyst on day 5.
Third ICSI22222Two viable embryos (both grade II 8 cell) were frozen on day 3 but failed to establish pregnancy.
Fourth ICSI66442Two viable embryos (a grade II 8 cell and a grade II 6 cell) were frozen on day 3; other embryos were further cultured but failed to form a blastocyst on day 5. The two frozen embryos were implanted later but failed to establish pregnancy.
Fifth ICSI44222One viable embryo (a grade II 10 cell) was frozen on day 3, and one was further cultured and formed blastocyst on day 5. The blastocyst was implant later but failed to establish pregnancy.
Sixth ICSI43332Two viable embryos (a grade II 8 cell and a grade II 7 cell) were frozen on day 3, and one embryo was further cultured but failed to form a blastocyst on day 5.
Family 4First IVF/ICSI332410102Two viable embryos (both grade II 9 cell) were frozen on day 3; other embryos were further cultured but failed to form a blastocyst on day 5. Two frozen embryos were implanted later but aborted on 22 weeks.
Second ICSI33220Two embryos were arrested at 5- to 6 cell stage on day 3
Third ICSI211413112Nine embryos were failed to form blastocysts on day 5; two embryos formed blastocysts on day 5 and were frozen. The two frozen embryos were implanted, and one was established pregnancy and parturition successfully. The baby is healthy.
Family 5First IVF146442Two embryos were arrested at 2–5 cell stage; one embryo (grade II 8 cells) was frozen, and others were further cultured, but only one was frozen for forming a blastocyst on day 5. Two embryos were implanted in twice, but both were failed.
Second ICSI55441One viable embryo (grade II six-cell embryo) was frozen, and the other three embryos were arrested at 4–5 cell stage.
Third ICSI88663Three embryos were arrest at 4–6 cell stage on day 3, three embryos, including two grade II seven-cell embryos and one grade II six-cells embryo, were frozen. Two seven-cell embryos were implanted and parturition successfully. Two babies are healthy.
Family 6(II-1)First ICSI261814110All embryos were arrested at the 2–4 cell stage on day 3.
Second IVF99770All embryos were arrested at the 4–6 cell stage on day 3.
Third ICSI1612990All embryos were arrested at the 2–4 cell stage on day 3.
Family 6(II-2)First ICSI1413990All embryos were arrested at the 2–7 cell stage on day 3.
Family 7First IVF161410100All embryos were arrested at the 2–4 cell stage on day 3.
Second IVF161512120All embryos were arrested at the 2–7 cell stage on day 3.
Third IVF43110The embryo was arrested at 3 cell stage on day 3.
  • ICSI, intracytoplasmic sperm injection; IVF, in vitro fertilisation.