TableĀ 2

Spindle status, polar body extrusion, fertilisation and ensuing embryonic development in wild-type and patient oocytes harbouring mutations in TUBB8

W.T.S176LI210VT238MV255MR262WT285PN348S27_33delT143 Dfs*12
SpindlePresentAbsentAbsentPresentAbsentAbsentPresentPresentPresent
Spindle morphologyNormal//Disorganised///Disorganised and low fluorescent intensityAberrant/
Polar body extrusionYes (100% oocytes)NoYes (4/15,26.7% oocytes)Yes (6/30,20% oocytes)NoNoNoYes (8/55,14.5% oocytes)Yes (1/33, 3% oocytes)Yes (1/19, 5.2% oocytes)
Fertilisation and embryo developmentYes/Normal fertilisation; Early embryonic arrestAbnormal fertilisation; Early embryonic arrest///Normal fertilisation; Early embryonic arrestAbnormal fertilisation; No cleavageAbnormal fertilisation; No cleavage
  • See text and figures 2 and 3 for details.

  • /, not applicable or no data were collected.